sirna-pgc-1α基因对mpp+诱导sh-sy5y细胞损伤的影响

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1、分类号：R741.02学校代码：10392学科专业代码：100204学号：2101003232福建医科大学硕士研究生毕业论文+siRNA-PGC-1α基因对MPP诱导SH-SY5Y细胞损伤的影响EffectsofsiRNAtargetingPGC-1αgeneonSH-SH5Y+cellinducedbyMPP学位类型：医学硕士所在学院：协和临床医学院研究生：司二旺学科、专业：神经病学导师：叶钦勇副教授研究起止日期：2011年11月至2013年3月答辩日期：2012年05月31日二○一三年五月目录英文缩略词表···············

2、········································1中文摘要···························································2英文摘要···························································4第一部分腺病毒介导siRNA抑制SH-SY5Y细胞PGC-1α基因表达的研究··6引言···························································6

3、1材料与方法···················································72结果·······················································193讨论·······················································22+第二部分沉默PGC-1α基因对MPP诱导SH-SY5Y细胞损伤的影响········24引言···············································

4、············241材料与方法···················································252结果························································293讨论························································32结论······························································34参考文献·······

5、·····················································35致谢······························································38综述······························································39英文缩略词表(Abbreviationsandacronyms)缩写英文全称中文全称DADopamine多巴胺GFPgreenfluorescentprotein绿色荧光

6、蛋白MOImultipleofinfection感染倍数+MPP1-methyl-4-phenyl-pyridineion甲基苯基四氢吡啶离子mRNAMessageribomicleicacid信使核糖核酸PBSPhosphate-burreredsaline磷酸盐缓冲液PCRPolymerasechainreaction聚合酶链式反应PDParkinsonDisease帕金森病PGC-1αperoxisomeproliferator-activatedreceptor1过氧化物酶体增殖物激活受体γalpha辅激活因子1αPMSFPhen

7、ylmethanesulfonylfluoride苯甲基磺酰氟RNAiRNAinterferenceRNA干扰siRNASmallinterferingRNA小干扰RNATHtyrosinehydroxylase酪氨酸羟化酶1+siRNA-PGC-1α基因对MPP诱导SH-SY5Y细胞损伤的影响中文摘要【背景】帕金森病(Parkinsondisease，PD)是一种中老年人常见的中枢神经系统退行性疾病,线粒体功能障碍在PD发病过程中发挥着重要作用，在与PD发病相关的众多环境与遗传因素中，线粒体功能障碍是一个共同的作用点。过氧化物酶体增殖物

8、激活受体γ辅激活因子1α(peroxisomeproliferator-activatedreceptor1alpha，PGC-1α)是一个多功能转录调节因子，广泛参与线粒体生物合成、糖脂代谢