板栗雄花序黄酮提取及纯化

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1、摘要板栗具有雌花少，雄花多的特点，由于雄花很多而且会耗费大量的营养物质，常常需要去除雄花。板栗花中含有丰富的对人体健康有益的黄酮类化合物，因此逐渐受到国内外的开发和利用。本文以板栗雄花花序为原料，对板栗雄花黄酮的提取、纯化分离工艺进行了探究，研究结果如下：主要试验结果如下：1提取工艺最终确定为微波辅助提取是板栗花黄酮最为理想的提取方法。最佳工艺参数为乙醇浓度70%，料液比1:40，功率180w，提取时间为2min，黄酮类化合物得率为5.20%。2食品加工处理方式应选用煮制处理2min为最佳煮制方案、蒸制处理的最佳蒸制时间同样为2min，0.14Mpa高压处理最

2、佳时间为15min、发酵处理最佳发酵时间为3天、最佳发酵量为5%。3原料的品种应选用燕红，因为燕红品种黄酮提取率要高于其他品种。从黄酮的含量上可以看出，燕红>怀九>燕平>怀黄。贮藏前的处理方式应选用阴干处理，从各个品种的趋势来看，黄酮提取效果阴干处理>冻干处理>烘干处理。最佳采收期应为盛花期，从黄酮提取率总趋势来看盛花期的板栗花品种>初花期的板栗花品种。4本文选用AB-8型大孔吸附树脂对板栗花进行分离提纯，其分离纯化板栗花黄酮的最佳工艺参数为上样液流速3mL/min、洗脱剂为70%的乙醇溶液浓度、洗脱液流速为2mL/min。关键词：板栗雄花序；黄酮；提取；分离

3、纯化ResearchofExtractionandSeparationandPurificationofFlavonoidsfromMaleInflorescenceAbstractMaleInflorescencewillwasteamountsofnutritionsinthattheMaleInflorescenceoftenneedstoberemoved.Chestnutpollenisrichinflavonoidswhichishelpfulforthehumanbeings’health.Thusitisgraduallybeingdevelo

4、pedandutilizedathomeandabroad.Theresearchhasbeendoneonflavonoidsextraction,purificationandseparationprocessestakingtheMaleInflorescenceasrawmaterial.Themainresultswereasfollow:1Microwave-assistedextractionforflavonoidsfromchestnutpollenwastheoptimumextractionmethod.Theoptimumconditi

5、onsofmicrowave-assistedextractionfromchestnutpollenwere:theethanolconcentration:70%;themicrowavepower:180w;theextractingtime:2minutes;thesolid-solventratio:1:40.Intheaboveconditions,theextractingratioofflavonoidsfromchestnutpollenwas5.2%.2Theoptimumfoodprocessingforflavonoidsextract

6、ionfromchestnutpollenwasasfollows:steamingprocessingtime:2min;boilingprocessingtime2min;pressureprocessingtemperatureonthe0.14MPa:15min;fermentationtime:3days;Yeastamount5%.3VarietiesofrawmaterialsshouldbeusedYanhong.TheextractingratioofYanhongflavonoidswashigherthanothervarieties.A

7、scouldbeseenfromtheextractingratio:Yanhong>Huaijiu>Yanping>Huaihuang.Theoptimumstorageprocessingshouldbeusedstorageprocessing.Ascouldbeseenfromtheextractingratio:driedprocessing>freezingprocessing>dryprocessing.Theoptimumharvesttimeforthechestnutpollenwasfull-bloomstage.Ascouldbesee

8、nfromtheextractingr