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1、动物学报 50(3):334-340,2004ActaZoologicaSinica冷驯化条件下长爪沙鼠血清瘦素浓度的变化及其3与能量收支和产热的关系33李兴升 王德华 杨 明中国科学院动物研究所,农业虫鼠害综合治理研究国家重点实验室,北京100080摘 要 为研究低温胁迫条件下长爪沙鼠的适应对策及瘦素对体重和能量平衡的调节作用,我们将7只成年雌性长爪沙鼠在5℃条件下驯化21d,另选7只作为对照,对体重、血清瘦素含量、体脂含量、摄入能、基础代谢率、非颤抖性产热等进行了测定。结果发现:1)5℃条件下长爪沙鼠的体重没有明显变化;2)5℃条件下长爪沙鼠的血清瘦素
2、浓度和体脂含量均明显低于对照组,且瘦素浓度与体脂含量呈显著正相关;3)5℃条件下长爪沙鼠的摄入能、基础代谢率和非颤抖性产热等显著高于对照。这些结果表明:长爪沙鼠在低温条件下产热能力和自身维持能量消耗都增加,能量摄入因此而增加;瘦素参与了能量平衡和体重的调节,但没有直接参与产热调节[动物学报50(3):334-340,2004]。关键词 长爪沙鼠 体重 瘦素 能量代谢 产热Effectsofcoldacclimationonbodyweight,serumleptinlevel,energymetabolismand3thermogenesisintheMo
3、ngoliangerbilMerionesunguiculatus33LIXing2Sheng,WANGDe2Hua,YANGMingStateKeyLaboratoryofIntegratedManagementofPestInsectsandRodents,InstituteofZoology,TheChineseAcademyofSciences,Beijing100080,ChinaAbstractTodeterminetheadaptivestrategyandtheroleofleptinintheregulationofbodyweighta
4、ndenergybalance,bodyweight,serumleptinlevel,bodyfatmass,energyintake,basalmetabolicrateandnonshiveringthermogenesisweremeasuredafter3weeksacclimationat5℃foradultfemaleMongoliangerbilsMerionesunguiculatus.Bodymasswasmonitoredevery3days.BodyfatcontentwasanalysedwiththeSoxhletmethod.
5、Energyintakewasmeasuredinfoodtri2als.Metabolictrialswereconductedwithclosed2circuitrespirometerandnonshiveringthermogenesiswasinducedbyinjec2tionofmass2dependentdosageofnoradrenaline.SerumleptinconcentrationwasmeasuredwithacommercialmultispeciesleptinRIAkit(LincoCompany,USA).Resul
6、tsshowedthat:1)nosignificantchangesinbodyweightwerefoundat5℃;2)serumleptinlevelsandbodyfatmasswerelowerat5℃thanthatofcontrol(23℃),thereisapositivecorrela2tionbetweenleptinconcentrationandbodyfatmass;3)energyintake,basalmetabolicrate,nonshiveringthermogenesiswerehigherat5℃thanthato
7、fcontrol.TheseresultssuggestedthatMongoliangerbilsenhancedthermogeniccapacityandincreasedtheirmaintenancecostduringcold2acclimation,thuscouldcausetheincreaseinenergyintake.Leptinpar2ticipatedintheregulationofenergybalanceandbodyweightforMongoliangerbils.However,itseemedthattherewa
8、snodirectrelationshipbetweenserum
