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ID:34045479
大小:2.68 MB
页数:58页
时间:2019-03-02
《牛蒡根有效部位的制备及通便作用的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、青岛大学硕士学位论文牛蒡根有效部位的制备及通便作用的实验研究姓名:赵建波申请学位级别:硕士专业:药理学指导教师:王春波;孙敬勇20120601摘要目的:制备牛蒡根6个有效部位;通过药理学实验筛选并确认牛蒡根药材的通便有效部位。方法:对牛蒡根进行生药学鉴定;采用溶剂提取法、萃取法、吸附法等按照极性大小对牛蒡根进行提取,分别制备出牛蒡水提物组、牛蒡醇水提物组、牛蒡醇提物组、牛蒡8096乙醇洗脱部位、牛蒡氯仿提物组、牛蒡石油醚提物组并计算收率;采用小肠运动法与炭末推进法药理实验,具体设计为:分别将小鼠随机分成7组,以生理盐水为对照组,牛蒡根醇提取
2、物、牛蒡根水提取物、牛蒡根醇水提取物、牛蒡根石油醚提取物、牛蒡根氯仿提取物、牛蒡根80%乙醇洗脱部位为实验组,各组分别每R灌胃给药1次,连续给予7d,测量小鼠首次排黑便时间、6h粪便数及推进率,运用SPSSl3.0软件进行数据分析,以P3、0.80min、6h粪便数51.8粒、推进率56.80%,下同)、醇提取物组(83.56min、42.5粒、46.51%)以及醇水提取物组(81.13min、44.8粒、48.1396)均优于空白对照组(100.99min、40.6粒、39.99%),其中水提取物组最为显著(P4、thepreparationandpurgeroleofexperimentsABSTRACTObjective:Preparationburdockrootsixeffectivepans;andthroughthepharmacologicalexperimentsconfirmburdockrootscreeningandcrudeaperienteffectiveparts.Methods:Burdockrootofonpharmacognosy;Solvent,extraction,adsorption,etcaccordingt5、othepolarityofburdockrootsizeextraction,,wepreparedburdockwaterextractingsgroup,burdockalcoholwaterextractingsgroup,burdockethanol-extractioncontentgroup,burdock80%ethanoltowashdownparts,burdockchloroformextractingsgroup,burdockpetroleumetherextractingsgroupandcalculationy6、ield;Themethodofsmallintestinemotorwithcarbonpushattheendofthepharmacologicalexperimentalmethod,andthespecificdesignfor:mouserandomlydividedinto7respectivelygrouptophysiologicalsalineascontrolgroup,burdockrootalcoholextract,burdockrootwaterextract,burdockrootextract,burdoc7、krootalcoholwaterpetroleumetherextract,burdockrootchloroformextraction,burdockroot80%ethanoltowashdownpartsoftheexperimentalgroup,thedailyfillthestomachrespectivelytomedicine,thecontinuousgive7d,measurementofmiceandtime,frstrOWblack6hfecesnumberandpushrate,usingSPSS13.0sof8、twaretoanalyze,toP<0.05saidwithastatisticalsignificance.Results:Usingthemethodofburdockro
3、0.80min、6h粪便数51.8粒、推进率56.80%,下同)、醇提取物组(83.56min、42.5粒、46.51%)以及醇水提取物组(81.13min、44.8粒、48.1396)均优于空白对照组(100.99min、40.6粒、39.99%),其中水提取物组最为显著(P4、thepreparationandpurgeroleofexperimentsABSTRACTObjective:Preparationburdockrootsixeffectivepans;andthroughthepharmacologicalexperimentsconfirmburdockrootscreeningandcrudeaperienteffectiveparts.Methods:Burdockrootofonpharmacognosy;Solvent,extraction,adsorption,etcaccordingt5、othepolarityofburdockrootsizeextraction,,wepreparedburdockwaterextractingsgroup,burdockalcoholwaterextractingsgroup,burdockethanol-extractioncontentgroup,burdock80%ethanoltowashdownparts,burdockchloroformextractingsgroup,burdockpetroleumetherextractingsgroupandcalculationy6、ield;Themethodofsmallintestinemotorwithcarbonpushattheendofthepharmacologicalexperimentalmethod,andthespecificdesignfor:mouserandomlydividedinto7respectivelygrouptophysiologicalsalineascontrolgroup,burdockrootalcoholextract,burdockrootwaterextract,burdockrootextract,burdoc7、krootalcoholwaterpetroleumetherextract,burdockrootchloroformextraction,burdockroot80%ethanoltowashdownpartsoftheexperimentalgroup,thedailyfillthestomachrespectivelytomedicine,thecontinuousgive7d,measurementofmiceandtime,frstrOWblack6hfecesnumberandpushrate,usingSPSS13.0sof8、twaretoanalyze,toP<0.05saidwithastatisticalsignificance.Results:Usingthemethodofburdockro
4、thepreparationandpurgeroleofexperimentsABSTRACTObjective:Preparationburdockrootsixeffectivepans;andthroughthepharmacologicalexperimentsconfirmburdockrootscreeningandcrudeaperienteffectiveparts.Methods:Burdockrootofonpharmacognosy;Solvent,extraction,adsorption,etcaccordingt
5、othepolarityofburdockrootsizeextraction,,wepreparedburdockwaterextractingsgroup,burdockalcoholwaterextractingsgroup,burdockethanol-extractioncontentgroup,burdock80%ethanoltowashdownparts,burdockchloroformextractingsgroup,burdockpetroleumetherextractingsgroupandcalculationy
6、ield;Themethodofsmallintestinemotorwithcarbonpushattheendofthepharmacologicalexperimentalmethod,andthespecificdesignfor:mouserandomlydividedinto7respectivelygrouptophysiologicalsalineascontrolgroup,burdockrootalcoholextract,burdockrootwaterextract,burdockrootextract,burdoc
7、krootalcoholwaterpetroleumetherextract,burdockrootchloroformextraction,burdockroot80%ethanoltowashdownpartsoftheexperimentalgroup,thedailyfillthestomachrespectivelytomedicine,thecontinuousgive7d,measurementofmiceandtime,frstrOWblack6hfecesnumberandpushrate,usingSPSS13.0sof
8、twaretoanalyze,toP<0.05saidwithastatisticalsignificance.Results:Usingthemethodofburdockro
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