超声激活原卟啉对h22小鼠肝癌细胞线粒体功能的影响

《超声激活原卟啉对h22小鼠肝癌细胞线粒体功能的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、withtheincreaseoftreatmenttimewhileprotoporphyringroupswerehardlyinfluenced．AndthemortalityonH22cellstreatedbyultrasoundcombinedprotoporphyrinwasmoreseriousthanthosetreatedbytheultrasoundalone．Finallythetreatmenttimewasdeterminedtobe30sinordertoreaserchitsmechanism．2．Thekillingeffectoftheul

2、trasoundcombinedprotoporphyrinonH22cellsWasexaminedatdifferenttimeafterthetreatment(0，1，2，3，4，5h)．TheresultsshowedthattherelativesurvivalratesofH22cellsinprotoporphyringroupshadunnotablychangesafterthetreatment,whiletherelativesurvivalratesinultrasoundgroupsandultrasoundcombinedprotoporphyr

3、ingroupsdroppedaftertreatmentdelay．Withthes锄eultrasoundintensity,andthedamageon1-122cellstreatedbyultrasoundcombinedprotoporphyrinWasmoreseriousthanthosetreatedbytheultrasoundalonewithin5houts．Sotheteststimewasdeterminedtobe0-5hoursinthefollowingexperiment．3．Morphologyobservationthroughsamp

4、lepreparationfordifferenttimethatprotoporphyrinc柚significantenhancethedamagedegreeofH22inducedbylow—intensityultrasoundandlingeringdamages，thesensitivekillingsitesofSDTaremembranesystemsandmitochondrionisoneofthemostsensitivesitetobedestroyed．4．Basedonformertests，thepermeabilitytransitionpo

5、re(FTP)oftumourcellsWasobservedbyUV-spectrometer,thefunctionofPTPgraduallydeclineaftertreatmentinultrasoundgroupandultrosondcombinedprotoporphyringroup．5．Wedetectedtheinfluenceofprotoporphyrincombinedultrasoundonmitochondrialmembranepotential(1abeledbyrodaminel23)．Contrastthecontrolgroup，th

6、emitochondrialmembranepotentialgraduallydescendaftertreatmentinultrasoundgroupandultrosondcombinedprotoporphyringroup．6．Theexpressionsofpro-apoptosisproteinsincludingBax，Smac,cytochromeCandcaspase-3wereexaminedbythemethodoftheimmunoeytochemistry,whichindicatedthattheexpressionsremainednegti

7、veinbothcontrolgroupsandprotoporphyringroups．hultrasoundgroups．fourpro—apoptosisproteinsexpressionstartedtoincreasethreehoursafterthesonication．However’theexpressionsstartedonehourafterultrasoundactivatingprotoporphyrintreatment,andmoreexpressionswereals