hif-1α与tfr mrna在脑白质软化动物模型中的表达及相关性研究

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1、分类号：R322.81学校代码：10392学科专业代码：100101学号：200901442福建医科大学硕士研究生毕业论文HIF-1α与TfRmRNA在脑白质软化动物模型中的表达及相关性研究ResearchontheexpressionofHIF-1αandTfRmRNAandtheirrelationshipinRatmodelswithHypoxic-ischemicPeriventricularLerkomalacia学位类型：医学硕士所在学院：基础医学院研究生：修丽莉学科、专业：人体解剖与组织胚胎学导师：王玮教授研究起止日期：2010年9月至2012年2月答辩日期

2、：2012年5月27日二○一二年六月万方数据万方数据目录中文摘要····························································1英文摘要····························································3前言······························································5第一部分脑室周围白质软化模型的建立1引言··············································

3、···········72材料与方法···················································73结果·······················································174讨论·······················································245结论·······················································28第二部分HIF-1α与TfRmRNA在脑白质软化动物模型中的表达及相关性1引言

4、·························································292材料与方法···················································303结果·······················································354讨论·······················································415结论················································

5、·······43参考文献··························································44英文缩略词表······················································47致谢····························································48综述····························································49万方数据HIF-1α与TfRmRNA在脑白质软化动物

6、模型中的表达及相关性研究中文摘要【目的】建立新生4天SD大鼠脑室周围白质软化模型（periventricularlerkomalacia,PVL）。在构建PVL模型大鼠的条件下，研究HIF-1α对TfRmRNA表达的调节，为缺氧缺血脑病发生机制与脑铁代谢之间关系的研究提供实验基础依据。【方法】1．4日龄新生SD大鼠通过随机数字表随机分为两组：PVL组和对照组；PVL组采用分离结扎双侧颈总动脉并予缺氧的方法建立脑室周围白质软化新生大鼠动物模型；对照组仅分离双侧颈总动脉，不予结扎及缺氧。采用HE染色法观察脑组织病理学改变，Westernblot法检测MBP蛋白表达，实验动物生

7、长发育及神经行为学等方法对动物模型进行评价。2．设立（1）对照＋HIF-1α组（2）对照＋空白组（3）PVL＋抗HIF-1α组（4）PVL＋PBS组；及（1）对照＋PBS组（2）对照＋抗HIF-1α组（3）PVL＋PBS组（4）PVL＋抗HIF-1α组；以Westernblot技术测HIF-1α表达，以Real-timePCR技术测TfRmRNA的表达。【结果】1．对4日龄新生SD大鼠进行双侧颈总动脉结扎并予以缺氧后，模型组与对照组大鼠相比，模型组大鼠生长缓慢（P<0.01），睁眼时间延迟（21.67±1.19dVS15.54