基于胆固醇的阳离子脂质材料的设计合成与其在基因转染中的应用-研究

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1、第四军医大学硕士学位论文3.2阳离子脂质体的表征...........................................463.3体外细胞毒性评价.............................................49第三部分阳离子脂质体的基因转染活性研究.............................531材料............................................................531.1仪器..................

2、.......................................531.2试剂.........................................................532方法............................................................542.1凝胶阻滞实验.................................................542.2体外基因转染活性的研究.....................

3、..................553结果与讨论......................................................573.1凝胶电泳分析.................................................573.2体外转染活性.................................................60小结.............................................................

4、69参考文献.............................................................70附录.............................................................76个人简历和研究成果...................................................99致谢............................................................100第四军医

5、大学硕士学位论文缩略词表缩略词英文全称中文全称DCCN,N'-dicyclohexylcarbodiamideN，N'-二环己基亚胺DC-Chol3β-[N-(N',N'-dimethylaminoethy3β-[N-（N'，N'-二甲基氨基乙l)-carbamoyl]cholesterol基）-氨基甲酰基]胆固醇DIADDiisopropylazodicarboxylate偶氮二甲酸二异丙酯DMAP4-dimethylaminopyridine4-二甲氨基吡啶DMSODimethylsulfoxide二甲基亚砜DNADeo

6、xyribonucleicacid脱氧核糖核酸DOPE1,2-dioleoyl-L-α-glycero-3-1，2-二油酰-L-α-甘油-3-磷脂酰phosphatidyl-ethanolamine乙醇胺EAEthylacetate乙酸乙酯FBSFetalbovineserum胎牛血清HN3hydrazoicacid叠氮酸1HNMRHydrogennuclearmagnetic核磁共振氢谱resonancespectrumIRInfraredspectroscopy红外光谱MFIMeanfluorescenceintensi

7、ty平均荧光强度MSMassspectrum质谱MTT3-(4,5-dimethylthiazole-2-yl)-2,3-(4，5-二甲基噻唑-2)-2，5-二5-diphenyl-tetrazoliumbromide苯基四氮唑溴盐NHSN-hydroxysuccinimideN-羟基琥珀酰亚胺PBSPhosphatebuffer磷酸盐缓冲液PDIPolydispersityIndex多分散系数PEPetroleumether石油醚PEGPolyethyleneglycol聚乙二醇-1-第四军医大学硕士学位论文pEGFPEn

8、hancedgreenfluorescent增强型绿色荧光蛋白质粒proteinplasmidPEIPolyethylenimine聚乙烯亚胺PPh3Triphenylphosphine三苯基膦RNARibonucleicacid核糖核酸TFATrifluoroaceticacid三氟乙酸