th17在肥胖哮喘小鼠气道炎症中作用及其和氧化应激关系论文

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1、Abstract恻嬲BackgroundInrecentyears，manystudieshaveshownthatobesitymayleadtoandaggravatethebronchialasthma(asthma)andtheirinteractionexiststoacertainextent，andtheglobalincidenceofasthmaandobesityarerisingtrendyearbyyear．ObjectiveToevaluatetheeffectofIL一17andoxidatives

2、tressontheairwayinflammationofobeseasthmamicewithhigh-f乱diets．DiscussPPARgammaagonistforthetreatmentofobeseasthmaticmiceeffectanditsrelationship、炳thThto17andtherelatedfactorsMathodsSixtyfemaleC57BL／6Jmicewererandomlydividedinto4groups，asthmagroupA，obeseasthmagroupB，

3、controlgroupCandtreatmentgroup(groupD)．GroupAweresensitizedandchallengedbyovalbum(OVA)andnormaldiets，GroupBweresensitized，challengedasgroupAandfed、析mhi曲-fatdiets，theGroupCweresensitized，challen,ged、Ⅳitllnormalsalineandfed丽tllnormaldiets．GroupDweresensitized，challeng

4、edasgroupBandPPARgammaagonistatomizationprocess．ThecellsinBALFwerecountandclassifiedafterstaining，IL一4，IL一6inBALFweredetectedbyELISA．IL一17，IL一23and8-iso-ProstaglandinF2a(8-iso-PGF2a)inlungtissueweredetectedbyEHSA。WAt，WAm，Pbm，astheremodelingindices，measuredinlungpath

5、ologicalsection．DeterminationtheexpressionofPPAR7bywesternblot。Alltheparameterswerecomparedbetween4groups．ResultsIncomparison谢mgroupC，theleukocytesandEOScountinBALF，IL-4andIL-6，WAftPbm．，WAm／PbminlungsectionwereincreasedaswellasIL-17and8-iso—PGF2ainlungtissueelevated

6、ingroupA，groupBandgroupD，whilethemaximumchangeswereobservedingroupB(P<0．05)．groupB>DgroupsfP<0．05)．WesternblotexaminationrevealedlungtissuenucleoproteinPPARgammaintheexpressionofthetreatmentgroupincreasedintheobeseasthmaticgroupPearsoncorrelationanalysisshowedthatIL

7、．17wereinpositivecorrelationwiththeIL．6inBALFand8-iso—PGF2a仁0．816and0．731，P<0．01)．ConclusionsThl7biasWasinvolvedintheairwayinflammationandoxidativestressofobeseasthmamicewithhigh-fatdiets．PPAR7agonistrosiglitazonesignificantlyimprovedairwayinflammation，airwayreconst

8、ructionandrevascularization．Postgraduate：WeiWang，DirectedbyProfessor：HuapingTangKeywords：Asthma；Obesity，oOxidativestress；IL一17；PeroxisomeP