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1、2011;31(5)南方医科大学学报(JSouthMedUniv)·817··基础研究·脂肪源干细胞移植对糖皮质激素性骨质疏松骨量影响的实验研究陶晖,余美春,杨会营,杨春,曲戎梅,戴景兴,余磊,原林南方医科大学人体解剖学教研室暨广东省组织构建与检测重点实验室,广东广州510515摘要:目的探索异体脂肪源干细胞(ADSCs)移植对糖皮质激素性骨质疏松(GIOP)大鼠骨密度和骨组织微结构的影响,为GIOP的治疗探索一种新的方法。方法雌性Wistar大鼠随机分为空白对照组(A组)、模型组(B组)、治疗对照组(C组)和细胞治疗组(D组)。利用醋酸泼尼松龙建模成功后,
2、C和D组分别通过尾静脉移植生理盐水和ADSCs。对各组大鼠作腰椎和股骨骨密度测量以及胫骨骨组织形态计量学分析。结果与A组相比,B组腰椎和股骨骨密度、骨小梁的相对体积、厚度和数量均明显降低,骨小梁的分离度和单位骨小梁破骨细胞数显著增加(P<0.05)。ADSCs治疗后,D组中的各项指标均明显改善,并与B组比较具有统计学差异(P<0.05),亦明显优于C组。结论ADSCs可以有效的改善大鼠GIOP,这为GIOP的治疗提供了一种新的方法,也为“筋膜学假说”提供了部分实验支持。关键词:筋膜学;脂肪源干细胞;骨质疏松;骨组织形态计量学中图分类号:R336;R34文献标
3、志码:A文章编号:1673-4254(2011)05-0817-05Effectofallogenicadipose-derivedstemcelltransplantationonbonemassinratswithglucocorticoid-inducedosteoporosisTAOHui,YUMei-chun,YANGHui-ying,YANGChun,QURong-mei,DAIJing-xing,YULei,YUANLinDepartmentofAnatomy,KeyLaboratoryofTissueConstructionandDetect
4、ionofGuangdongProvince,SouthernMedicalUniversity,Guangzhou510515,ChinaAbstract:ObjectiveToinvestigatetheeffectsofsystemictransplantationofallogenicadipose-derivedstemcells(ADSCs),themainundifferentiatedcellsinthesupporting-storingsystembasedonthefasciologyhypothesis,ontheboneminera
5、ldensity(BMD)andhistomorphometryinratswithglucocorticoid-inducedosteoporosis(GIOP)rats,andexploreanewtherapeuticapproachforosteoporosis.MethodsFortyfemaleadultWistarratswererandomizedequallyintoblankcontrolgroup(A),modelgroup(B),controltreatmentgroup(C)andtreatmentgroup(D).Ingroups
6、B,C,andD,osteoporosiswasinducedbyinjectionofprednisolone(8mg/kg)viathetailvein3timesaweekfor12consecutiveweeks.Aftersuccessfulestablishmentofosteoporosis,allogenicADSCs(3×106/ml)weretransplantedviathetailvein.TheBMDattheL3-L5levelsandoftherightfemursweredetected,andthehistomorphome
7、tryoftherighttibiaswasanalyzedinalltherats.ResultsAfterprednisoloneinjection,theBMDofL3-L5vertebraeandtherightfemurs,thepercenttrabeculararea,trabecularthicknessandtrabecularnumberoftherighttibiaswereallobviouslyloweredwhilethetrabecularseparationandosteoclastnumberincreasedingroup
8、BascomparedtothoseingroupA