k562白血病细胞分化过程中线粒体铁蛋白表达情况分析

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1、●四川大学临床医学硕士专业学位论文TheExpressionofMitochondrialFerritin(MtF)inK562LeukemicCellsduringInducedCellDifferentiationPostgraduate：SunleiTutor：associate—professorGaojuAbdtractINTRODUCTIONIronisessentialtocellproliferationandsurvival，foritserversasacofactorofmanyenzymespar

2、ticipatinginsuchmetabolicprocessesasDNAbiosynthesis，hemesynthesisandelectrontransfer．Therefore，cellironhomeostasishastobefinelyregulated,andabnormalitiesinironmetabolismalecloselyrelatedtoavarietyofpathophysiologicconditions，includingimmuneinjuriesandtuinorformat

3、ion．Mitochodria,avitalcellorgandie，playsveryimportantrolesinhemeandiron-sulfurclusterproteinbiosynthesis．Ironhomeostasiswithinthisparticularcellcompartmentmustbealsofreelyregulated．Thishelpstopreventbothinadequateironsupplytovariousmetabolicprocessesandexcessivei

4、ronaccumulation,whichisimplicatedintheproductionsoffreeoxygenradicalsandthusoxidativestressdamage．Mitochondrialferritin(MtF)，anewplayerinironmetabolism，firstidentifiedin2001，isldghlyhomologoustoferritinbothstructurallyandfunctionally．MtFgeneislocatedonthelongarln

5、ofchromosome5(5q23．1)andMtFproteiniscomposedof185aminoacids．Preliminarystudieshavesuggested4●四川大学临床医学硕士专业学位论文thatMtFmightp“yveryimportantrolesintheregulationofmitochondrialironhomeostasis．Leukemiccells，jnstlikeothermalignantcells，demandmoreironfortheirgreaterprol

6、iferationpotential．BasedonthepossiblerolesofMtFincellironmetabolism，wepredictedthatitmaybeimplicatedinleukemiccellproliferation．HerewestudiedthemRNAexpressionsofMtF,transfererinreceptor1fire：)andferfitininK562leukemiccellsduringTPAandATRA-inducedcelldifferentiati

7、ons．OBJECTIVESTostudytheexpressionpatternsofmitoehondrialferritin(MtF)，transferrinreceptorl(Tml)andferritin(Fn)mRNAsinK562leukemiccellsduringTPAIATRA-inducedcelldifferentiationandtoexploretheinterrelationshipbetweentheexpressionlevelsoftheseironmetabolism-related

8、molecules．METHODSK562cells(5x105／m11wereculturedwithorwithoutCO．incubationwithTPA(fmalconcentration16nmol／L)／ATRA(finalconcentrationIIjtmol／L)．After24，72and120