穴位埋药对脑缺血再灌注大鼠海马肿瘤坏死因子和核因子-κb的影响

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1、湖北中医学院2005级硕士研究生毕业论文穴位埋药对局灶性脑缺血再灌注大鼠肿瘤坏死因子和核因子NF-1cB的影响ABSTRACTObjectiveOnthebasisofcomprehensivetherapywithacupunctureforapoplexyatthepresenttime，combiningmodernpharmacologicalresearchtopreparecollagentetramethylpyrazineSUStained—eleasedose，toresearchtheeffectof

2、embeddingmedicineinDazhuiandNeiguanacupointSontheinfarctvolumeanditSregulatingeffectontumornecrosiSfactor—o【andnuclearfactorkappaB．MethodsTotaIly160healthySDratswereselectedanddividedrandomlyinto6groups，Thegroupsincludednorm1group，shamgroup，mode卜24hgroup，model72h

3、group，model120hgroup，abdominalcavityinjettion24hgroup，abdominalcavityinjection72hgroup，abdominalcavityinjection120hgroup，electroacupuncture24hgroup，electroacupuncture72hgroup，electroacupuncture120hgroup，embeddingmedicineinacupointS24hgroup，embeddingmedicineinacup

4、oints72hgroup，embeddingmedicineinacupointS120hgroup，with10ineachgroup．ThemodelofischemiareperfusionofcerebralmiddlearterywasestablishedbythemodifiedthreadOCtlusionmethod，thenembeddingmedicineinDazhuiandNeiguanacupointswithC01lagentetramethylpyrazineSUStained—rele

5、asedose．4ratsofeachgroupweredecollateddirectlytoinvestigatetheexpressionofTNF一仅inhippocampusbyradioimmunoassayandtheexpressionofNF一1cB—p65proteininhippocampuswereassayedbyimmunohiStochemistry：andother6ratswerereperfusedwith4％paraformaldehydeafterbeinganaesthetize

6、dby10％chloralhydrate，andthentakenoutthebrainrapidly．ThebraintisSUe2mmawayfromthechiasmawascutfrontally，themiddlepartleft，immersedinhardparaffin．UsedimmunhiStochemiStrySABCmethodtoinvestigatetheexpressionofNF一1CB，andtheeffectofa11theaboveindexof3湖北中医学院2005级硕士研究生毕业

7、论文穴位埋药对局灶性脑缺血再灌注大鼠肿瘤坏死因子和核因子NF-KB的影响electro—acupuncturewereobserved．TheentiredatumwasanalyzedbyStatiStics．ResultS1．MACOandreperfusioninjurycandamagetheratsactivitydiStinctly．ThenervesymptomsofabdominaIcavityinjectiongroup，electro—acupuncturegroupandembeddingmedic

8、ineinacupointSgroupwereamelioratedobviously；therewasasignificantdifferencebetweenmodelgroupandeachtherapygroup，andtheembindingmedicineinacupointsgroupcanimprov