散血草对血管瘤裸鼠模型瘤体生长的影响及其机制探讨

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1、中图分类号：R6；R726硕士学位论文散血草对血管瘤裸鼠模型瘤体生长的影响及其机制探讨院（系）、所临床医学院研究生姓名徐勇学科、专业外科学导师姓名彭强二Ο一二年四月目录1散血草对血管瘤裸鼠模型瘤体生长的影响及其机制探讨„„„„„„„„„„„„„„„„„„„„„„„„11.1中文摘要„„„„„„„„„„„„„„„„„„„„„11.2英文摘要„„„„„„„„„„„„„„„„„„„„„31.3前言„„„„„„„„„„„„„„„„„„„„„„„61.4材料与方法„„„„„„„„„„„„„„„„„„„„71.5结果„„„„„

2、„„„„„„„„„„„„„„„„„„121.6讨论„„„„„„„„„„„„„„„„„„„„„„„171.7结论„„„„„„„„„„„„„„„„„„„„„„„221.8参考文献„„„„„„„„„„„„„„„„„„„„„231.9英汉缩略词对照表„„„„„„„„„„„„„„„„„262致谢„„„„„„„„„„„„„„„„„„„„„„„273婴幼儿血管瘤的治疗进展（综述）„„„„„„„„„„28散血草对血管瘤裸鼠模型瘤体生长的影响及机制探讨摘要目的：通过建立血管瘤裸鼠模型，观察散血草对血管瘤裸鼠模型生长的影响并探讨其可能

3、的作用机制。方法：采用组织块移植法，在无菌条件下取婴幼儿增生期毛细血管瘤手术标本切成小块，植入裸鼠皮下，制成婴幼儿血管瘤裸鼠异种移植动物模型。移植60天后，取移植成功的实验动物模型建立随机对照实验，分为4个实验组，分别给予以下不同干预措施：散血草组在血管瘤瘤体表面外用自制的散血草搽剂每日一次；激素组在血管瘤瘤体内均匀注射曲安奈德注射液0.05ml(4mg／ml)一次；散血草加激素组瘤体表面外用散血草搽剂，并在瘤体内注射曲安奈德注射液0.05ml(4mg／ml)一次；对照组瘤体表面外用对照物每日一次。给予不同干预措施

4、后，通过对血管瘤裸鼠模型瘤体体积的测量，监测血管瘤在裸鼠模型体内的生长情况；用药后15天处死实验裸鼠模型，取出血管瘤组织制成切片，HE染色进行组织学分析，并作免疫组织化学（S-P）染色检测HIF-1α、bcl-2和Ki-67表达的阳性指数，分析血管瘤在裸鼠模型体内的增殖和凋亡情况。使用SPSS14.0统计软件对所有实验结果进行统计学分析，以P<0.05为有显著性差异。结果：制作的血管瘤裸鼠模型生长良好，血管瘤瘤体体积大小均匀，经对照物、散血草、散血草加激素、激素处理15天后，对照组裸鼠模型血管瘤瘤体体积继续增大，标

5、本大体及镜下观测血管瘤瘤体呈增生期改变；散血草组、激素组、散血草加激素组裸鼠模型血管瘤瘤体体积与对照组相比均有缩小，各组之间血管瘤瘤体体积也均有显1著性差异，组织标本观察到血管减少，瘤体萎缩、退化，血管腔闭塞、破裂，血管间纤维脂肪组织浸润，其中散血草加激素组最明显，激素组次之，散血草组血管瘤瘤体缩小程度最小。散血草组、激素组、散血草加激素组HIF-1α、bcl-2和Ki-67阳性指数与对照组相比均有减少，而且各组间相比也均有显著性差异，HIF-1α、bcl-2和Ki-67阳性指数在散血草加激素组减少最明显，激素组次

6、之，散血草组瘤体减少程度最小。结论：散血草外用可以抑制血管瘤裸鼠移植模型瘤体的增殖；散血草外用与糖皮质激素(曲安奈德)联合应用，对血管瘤裸鼠移植模型瘤体增殖的抑制作用强于单用散血草或糖皮质激素；单用散血草对血管瘤裸鼠移植模型瘤体增殖的抑制作用不及单用糖皮质激素；散血草外用对血管瘤裸鼠移植模型瘤体增殖的抑制作用，可能与下调HIF-1α、bcl-2和Ki-67的表达水平有关。关键词：散血草；激素；血管瘤；裸鼠2TheexploreofmechanismtotheAffectofHemangiomanudemousemo

7、delbyAjugadecumbensThunbAbstract：Objective:OfscatteredhemangiomanudemousemodelofvasculartumortherapeuticeffectandmechanismofactionbyAjugadecumbensThunb,throughtheestablishmentofhemangiomanudemicemodel.Methods:Usingtissuetransplantation,takeinfantsproliferative

8、phasecapillaryhemangiomasurgicalspecimenswerecutintosmallpiecesundersterileconditions,implantedsubcutaneouslyintonudemice,madeofinfantilehemangiomasinnudemicexenograftanimalmodels.