抑瘤素m在膝关节骨关节炎中的表达

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1、lb尔』(学颁十学付论文立即用4％多聚甲醛阎定。取一80℃保存的滑膜组织标本约5册x5mmx5mm太小，J；ljTr{Z01一步法提取组织中的总RNA，然后逆转录获得eDNA，以(；APDH为内参．采用实时定量PCR技术(qRT—PCR)扩增目的基因和内参蘑因。经d％多聚甲醛同定的滑膜标本48小时后常规脱水、透明、包蜡。然后切片机切成5um厚的组织切片。两步法免疫纽化榆测备组滑膜组织中OSY的表达情况。结果：应用相对定量分析浊测得各组OSM的表达量，轻微病变纽基冈表达水平高于对照组差异无统计学意义(尸)n晒)；病变

2、严重组()sM基因袭选明显增多．与轻微病变组、对照组比较差异有统计学意义(P

3、病过程中一J能起重要作用。关键词：骨关节炎；滑膜；抑瘤素M：膝关节TheexpressionofoncostatinMinkneeosteoarthritisPostgraduate：ZHANGPengMajor：OrthopaedicsPutor：Prof．SUNShujABSTRACTObjectiveOsteoarthritisisakindofcommonmultiple—factor—relateddisease．"xilhitsincidenceincreasesovertheyearsItspredo

4、minantpathologicalchangeisthearticularcartilagedegenerationandosteophytelbrmationcomplexetiologyandpathogenesishavenOlbeenfullyexplainedRecentresearcheshaveconfirmedthatthesystemicorlocalchangesofcellfactorsleadtodestructionofarticularcartilagematrixandbonehyp

5、erplasiaAsisknown，IL—IandIL一6arecrucialregulatorsofinflammation．whichalsoinvolveintheprocessofOAOncostatinMisamemberofII一6family．anditcouldregulatetheactivityofosteoblastReseardlesfocusedonthefunctionsandmechanismsofOSMinOAhavenotbeenstainedyetInthisstudyweaim

6、todetecttheexpressionofoncostatinMinthesynoviumofOApatient．andtoexploretherelationshipbetweenoncostatinMand()AMaterialSandMethods1Wecollected51specimensfromOAandmeniscusinjurypatientsBasedonosteoarthritisdiagnosisstandardreferenceX·rayAItmanandAhibackX—rayclas

7、sificationmethodthespecimensaredividedintothreegroupsGroupA(SeriousOAGroup)：33patients(33knees)，male8cases(8knees)，female25cases(25knees)，aged47·75years，meanage589years，andkneelesionsX—rayclass3above；GroupB(SlightOAGroup)10patientsinOAmildgroup(10knee)，malein1

8、(Iknee)，female9cases(9knees)aged42—58yearsoldmeanage493yearsthecourseoflessthan3years，andkneeLesionsX—rayclassificationlevelI．2GroupC(SufferedfromlateraldiscoidwithoutOA)8caseswith