igf-1对大鼠局灶性脑缺血再灌注后vegf、bfgf表达的影响与意义

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1、中文摘要间点bFGFmRNA表达均较同时间点高，P<0．05，有统计学意义。7、HE染色，光镜下观察，假手术组脑组织结构无明显异常。缺血再灌注24h正常神经细胞数减少明显，神经元空泡变性多，可见细胞核固缩，组织疏松，细胞间隙增大。IGF。1治疗组脑组织损伤程度较轻，皮层神经元细胞空泡变性较少，间质水肿轻，正常细胞数增多。结论：l、本实验通过侧脑室注入外源性IGF．1，明显改善了大鼠脑缺血再灌注后的神经功能，证明IGF．1对缺血再灌注损伤有保护作用。2、脑缺血后可诱导VEGF、bFGF基因及蛋白表达增高，是自我保护机制的一种反应。3、IGF．1可以使脑缺血再灌注后脑组织中VEGF、bFGF的

2、表达进一步增高。4、IGF．1对脑缺血的保护作用可能是通过增加脑组织中VEGF、bFGF基因及蛋白表达而实现的。5、本实验探讨了IGF．1对脑缺血的保护作用及可能机制，为临床应用IGF．1治疗脑缺血损伤提供了理论依据。关键词：缺血再灌注；胰岛素样生长因子．1；血管内皮生长因子；碱性成纤维生长因子；基因表达英文摘要TheeffectandsignificanceoflGF一1ontheexpressionofVEGFandbFGFaftercerebrallSCilemlcreperlusloninrats●_●n■●ABSTRACTObjective：Ischemiccerebrovasc

3、ulardiseaseiSthecommoncentralnervoussystemdiseaseanditsrateofmortalityanddisabilityisveryhigh．Ischemiccerebrovasculardisease，heartdiseaseandmalignanttumorarethethreediseasesleadingtodisabilityinmostcountriesandseverelythreatenhumans’healthy．Howtoimprovethecerebralbloodflowoftheischemicbraindamagea

4、ndpromotetherepairandregenerationofthedamagedneuronhavebecomethefocusanddifficultyofmanystudies．Therefore，researchonthenervegrowthfactorsandvasculargrowthfactorscausedgreatextraattention．Currently,insulin—likegrowthfactor-1(IGF一1)、vascularendothelialgrowthfactor(VEGF)andbasicfibroblastgrowthfactor

5、(bFGF)arethemostlypopularstudiedfactors．Alongwiththecontinuousdeepeningofthestudy,endogenousIGF一1hasbegantobeusedintheexperimentofischemiccerebrovasculardisease，butitisstillnotclearabouttherelationshipbetweenIGF-1andtheexpressionofVEGFandbFGFandtheexactneurotrophicprotectivemechanismofIGF一1afterce

6、rebralischemiaSOfar．Theratmodelofmiddlecerebralarteryocclusion(MCAO)andreperfusionwasestablishedwithsutureembolimethod．5英文摘要AfterinjectingIGF-1vialateralcerebralventricle，theIGF一1’SeffectontheexpressionofVEGFandbFGFbothongeneandproteinlevelwasobservedthroughRT-PCRandimmunohisto—chemistrymethods．In

7、thisstudy,wepreliminarilydiscussedthepossiblemolecularmechanismofIGF-1’sprotectiveeffectstowardscerebralischemicdamageandprovidedanewtheoreticalbasisforIGF一1’Sfurtherclinicalapplicationandanewwayfortheclinicaltre