补阳还五汤联合mscs移植对大鼠脑缺血再灌注损伤模型vegf和ki-67表达的影响

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1、补阳还五汤联合骨髓间充质干细胞移植对大鼠脑缺血再灌注模型脑组织VEGF和Ki-67表达的影响治疗组与对照组比较P＜0.01；益气活血方联合MSCs治疗组与MSCs移植治疗组比较P＜0.05。3.空白组脑组织中Ki-67几乎不表达，对照组、MSCs移植治疗组和益气活血方联合MSCs治疗组均可见到Ki-67表达，MSCs移植治疗组与对照组比较P＜0.05，益气活血方联合MSCs治疗组与对照组比较P＜0.01；益气活血方联合MSCs治疗组与MSCs移植治疗组比较P＜0.05。结论：1.采用密度梯度离心法获

2、取骨髓间充质干细胞（MSCs），将细胞经过传代培养使细胞得到扩增和纯化的MSCs。2.短暂性脑缺血再灌注损伤动物模型的制备采用改良线栓法取得了较好效果，神经行为学评分理想，症状很接近人表现。其简便、易行，容易操作，很具有实用性，是制作脑缺血再灌注损伤动物模型理想的方法。3.应用免疫组化染色法证明了益气活血方联合MSCs移植治疗短暂性脑缺血再灌注损伤可能通过诱发促进脑内VEGF和Ki-67的高水平表达，激活内源性神经保护机制，使它在保护病灶周围神经元、促进细胞增殖和组织修复中可能发挥着重要作用。关键词

3、：补阳还五汤；骨髓间充质干细胞（MSCs）；脑缺血再灌注；免疫组化；VEGF；Ki-67-2-AbstractTheeffectofBuyanghuanwusoupcombinedwithMSCstransplantationontheexpressionofVEGFandKi-67ofthecerebralischemia-reperfusionmodelinrats.HanXueyong(InternalMedicineofTraditionalChineseMedicine)Directedb

4、yprofessorZhangYunkeAbstractObjective：ToinvestigatetheeffectsandtheprotectivemechanismsofSaponinsofbuyanghuanwusoupcombinedwithbonemarrow-derivedmesenchymalstemcelltransplantation.SDratsMSCswereculture.Themodeloflocalischemia-reperfusioninratswasestabl

5、ishedwiththesutureoccludedmethodinventedbyZeaLongaandimprovedproperlyinourlab..ThendetectedtheVEGFandKi-67ofratesafterischemiareperfusionrespectivelybyimmunohistoch-Emistricalmethod,andObservatetheresPathologicalmanifestations.Toinvestigatethemechanism

6、sofBuyanghuanwusoupcombinedwithbonemarrow-derivedmesenchymalstemcelltransplantationrepaircerebralischemia-reperfusioninjuryinratbloodvesselsandlesions.Method:ThehealthyadultSDratsincleangradeareremovedthefemursandtibiasundersterileconditions.Thenwecanm

7、akeoutofthebonemarrowbyDMEMculturemediumandfilteritby200-meshsteelnet.NextobtainthebonemarrowmononuclearcellsbydensitygradientcentrifugationandwashtwicebyPBS.ThesedimentsshouldbejoinedinDMEM10mlcontaining10%FBSculturemedium，thengiveitgentlewindandpercu

8、ssionandmakeitcellsuspension,counting,dilution.ItshouldbeinoculatedintothebottleofT-75culturewithdesigndensity.Thebottlecanbeplacedintheincubatorwith37℃,thevolumefractionof5%CO2andsaturatedhumiditytoprimaryculturecell.Whenthebottomofthe