加味补阳还五汤对局灶性脑缺血再灌注大鼠脑组织bdnf、ngf表达的影响

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1、group．Preparationfocalcerebralischemicmodelbysuturemethodocclusingtheleftmiddlecerebralartery．Theeonditionsshamoperationisthesametoothertwogroupsbesidesnotocclusingbythread．2．BraintissuesliceAftersuccessfullyconstractingthemodel，ratsaresacrificedatdifferenttimepoint．Thebrainbehindopticchiasmaistak

2、enout，andthensituatedin4"C，4％paraformaldehydeprosphatebuffersolutionfor24h．Regulardehydration，waxing，embeddingandpressinginto71xmthickcoronalslice．Thenplacingontheglassslideandpreservingitin60"(2temperatureboxfor2hours．3．ImmunohistochemistrystainingUsingSABCmethod，andimmunohistochemistrytechonolog

3、y,observingtheimmunoreactivecellofBDNFandNGF，statisticallingthenumberofimmunoreactivecell、^，imimageanalysissoftware．4．AnalysisofthedataTheresultisdisplayedasMean+standarddevition，P<0．05isconsideredasstatisticallysignificantbyt-test．ResultsComparedwiththesham-operatedgroup，theexpressionofBDNFandNGF

4、increasedat12hours．24hoursand48hoursafterischemia(P<0．01)．Compared谢t}1theischemiagroup，theexpressionofBDNFincreasedatdifferentstagesafterischemia(P<0．01)．TheexpressionofNGFisalso(P<0．05)，andreachedtheclimaxafter48hours．ConclusionsTheBDNFandNGFhasincreasedinfocalcerebralischemia／reperfusionratsafte

5、rusingModifiedBuyanghuanwuDecoction，promotingtherecoveryofinjuriedneurons·KevwordsCerebralischemia／reperfusion,BDNF，NGF，BuyanghuanwuDecoction4NGFnervegrowthfactorMCAOCCAICAECAICVDMiddlecerebralarteryocclusioncommoncarotidarteryintemalcarotidarteryexternalcarotidarteryischemiccerebralvasculardiseas

6、e5神经生长因子大脑中动脉栓塞颈总动脉颈内动脉颈外动脉缺血性脑血管病·论文·加味补阳还五汤对局灶性脑缺血再灌注大鼠脑组织BDNF、NGF表达的影响刖声缺血性中风是以猝然昏仆，不省人事，半身不遂，口眼歪斜，言语不利等为主症的病证，西医称之为缺血性脑血管病(ICVD)，是严重危害人类健康的疾病之一，具有高死亡率和高致残率。近几年来，随着社会人口的老龄化以及人们生活水平的提高和生活方式的改变，其发病率呈逐年上升趋势【lJ，给社会和家庭带来沉重的负担，对其防治与机理研究一直是当今医学界研究的热点之一。中医理论认为风、火、痰、瘀、气、虚是中风的病理因素，气滞血瘀是其急性期基本病机之一。随着现代医学对

7、中风认识的不断深入，在发病机制上提出了兴奋性氨基酸毒性作用、氧自由基损伤、炎症、细胞凋亡等学说【21，明确了脑梗死缺血半暗带的概念和缺血再灌注损伤级联反应学说，认为半暗带是级联反应起作用的地方，虽然该区细胞结构存在，但神经功能受损。脑源性神经营养因子(brain．derivedneurotrophicfactor,BDNF)及神经生长因子(nervegrowthfactor,NGF)可维持神经元的存活及修复受损神经【3】