音猬因子复合骨髓间充质干细胞—支架复合物修复大鼠股骨缺损

《音猬因子复合骨髓间充质干细胞—支架复合物修复大鼠股骨缺损》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、江苏大学硕士学位论文Sonichedgehogcombinedwithmarrowmesenchyrealstemcells-·------scaffoldcompoundfortherepairofratfemoralbonedefects．[Abstract][Objeetive]：TorepairtheratfemoralbonedefectsinSDratswithnanohydroxyapatite／collagen(nHAC)andsonichedgehog(Shh)CO—culturedwit

2、hmarrowmesenchymalstemcells(MSCs)，comparedtheeffectoffemoraldefectrepair,preliminarystudyfortreatmentofbonedefectswaysoftissueengineering．[Methods]：Animalmodelsoffemoralbonedefectwereestablishedin30Sprague—Dawleyrats．Tlleyweredividedinto3groupsrandomly：Ing

3、roupA，defectwasfilledwithnone；ingroupBwithnHAC—MSCscompound；ingroupCwithMSCs／Shh—nHACcompound．MSCswereisolated，purified，cultured，identifiedandobservedcontinually．Scaffoldmaterialswerecutintoblockswiththesizeo勋6mmx5mmx5mm．Cellsofthe3rdgenerationswereCO—cult

4、uredwithMSCs／Shh-nHACinvitro．Thecompositegraftsweretestedbyscanningelectronmicroscope．Abonedefect(5mminlength)wascreatedattwofemoralineachmouse．Thecompositegraftswereimplantedintosegmentalfemoraldefectsinratsthroughopenoperation．Thecurativeeffectwasevaluat

5、edbygeneralobservation，radiographicexamination，histologicanalysisat6and12weeks．[Results】：After14days’compositecultivationoutsides，MSCsexhibitednewcellgrowthbyscanningelectronmicroscope．AtSixweeksaftertheoperation，whilegroupAshowedobviousbonedefects，whichre

6、mainedtobeseenafteranother6weeks，groupBshowedcloudynewbomosteotylus，whichgrewintogreatquantityafterIII江苏大学硕士学位论文another6weeks，butstillnotinbone-mouldingprocess，andgroupCshowedthedegradationoftheShh—nHACscaffoldandafewnewboneformationaswellasthecloudbetween

7、bonedefectsbyradiographicexamination，whichgrewintocontinuousosteotylUSandsmoothofbonemarrowcavityandbonetrabeculainsteadoftheplantedMSCs／Shh-nHACcompound．Thenewboneformationwassignificantlydifferentbetweentheexperimentalandthecontrolgroupsbyradiographicexa

8、minationat6and12weeksafteroperation(P<0．05)．Andthespeedandquantityofnewboneformationwasalsosignificantlydifferentbetweentheexperimentalandthecontrolgroupsbyhistologicanalysis(P<0．05)．[Conclusion]：MSCsareideal