约氏乳酸杆菌bs15对小鼠脂肪沉积和非酒精性脂肪肝的影响-(6089)

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1、万方数据EffectofLactobacillusjohnsoniiBSl5OilfataccumulationandNAFLDinhigh-fatdietinducedobesitymiceABSTRACTToinvestigatetheeffectofLactobacillusjohnsoniiBS15onobesityandnonalcoholicfattyliverdisease(NAFLD)，120maleICRmiceweredividedintotwogroupsandadministeredwitheitherPBS(PH7．0)烈Dgroup)orBS15(dai

2、lyamountsofeither2×107cfu，L-BSl5group；or2×108cfu，H—BSl5group)bygavagethroughouta17-weekexperimentalperiod．Themicewerefedwithnormalchowdiet(NCD)fiveweeksbeforetheexperimentalperiod．After35days，ThePBSgroupwasalsodividedintoNDandHFDgroups．ThedietoftheHFDandBS15groupswaschangedintohighfatfortheres

3、toftheweeks。Thesamplingtimepointswas35，63，91，119daysoftheexperiment．Resultswereasfollows：1．ThebodyweightofthemiceinHFDgroupwassignificanthigherthanNDgroupattheendoftheexperiment．BothdosesofBS15supplementationsuppressedtheincreaseofbodyweightduetothehigh-fatdietconsumption．2．Liversectionfrommic

4、eofHFDgroupexhibitedvisibleintracellularvacuolizationwhichmarkedlipidaccumulation，butthedegreeofhepaticsteatosiswasappreciablyattenuatedbythetreatmentofBSl5．3．Thedetectionofliverapoptosisbyflowcytometry(FCM)showedthatthepercentageofapoptoticcellsinliverofHFDgroupwassignificantlyincreasedcompar

5、edwithNDgroup，andthegrowthtrendwasinhibitedbythesupplementationofBSl5．4．TheresultsofRT—PCRquantificationofmicrobialcecalcontentshowedthatthesupplementationofBS15significantlyincreasedtheabundanceofLactobacillusspp．，L．johnsonii，andBacteroidetes，andloweredtheabundanceofEnterobacteriaceaefamilyan

6、dtheratioofFirmicutes}Bacteroidetes．Thegutfloradisturbanceinducedbyhigh—fatdietwasattenuatedbythetreatmentofBS155．LipidmetabolismgeneexpressionwasdetectedthoughRTrPCR．ComparedwithNDgroup，themRNAlevelsofACC1，FASandPPARyweresignificantly¨I万方数据increasedinHFDgroup，thegenelevelofFIAFwasmarkedlyredu

7、ced．AfterthetreatmentofBS15，themRNAlevelsofACC1，FASandPPA脚inBS15-groupwereapparentlyloweredincomparisonwithHFDgroup，andthelevelofFIAFwassignificantlyincreased．6．ThegutpermeabilityofmiceinHFDgroupwassignificantlyincomparisonwithNDgroup．A