复方芪参提取物抗瘢痕疙瘩的tgf-βsmad信号转导及mapk通路调控机制

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1、目录缩略词表...........................................................................................................................1中文摘要………………………………………………………………….......................3英文摘要………………………………………………………………….......................91.前言…………………………………………………………………....................

2、...182.材料与方法……………………………………………………………...................232.1材料…………………………………………………………………......................232.1.1细胞.......................................................................................................................232.1.2药品........…………………………………………………………..........

3、.............242.1.3主要仪器…………………………………………………...................................242.1.4主要试剂及抗体………………………………………………….......................252.1.5主要试剂配制…………………………………………………….......................272.2方法…………………………………………………………………......................302.2.1体外分离培养成纤维细胞…….....................

4、......................................................302.2.2体外细胞划痕实验观察CASE对瘢痕疙瘩成纤维细胞移行能力的影响….....302.2.3CASE对瘢痕疙瘩成纤维细胞内TGF-/Smad信号转导途径的调控作用.......312.2.3.1细胞培养与分组................................................................................................312.2.3.2免疫沉淀与westernblot

5、法检测CASE对TGF-1诱导的Smad2/3磷酸化及Smad2/3/4复合物表达的影响.......................................................................................312.2.3.3细胞免疫荧光染色法检测CASE对TGF-1诱导的磷酸化Smad2/3转位入核的影响......................................................................................................

6、.......................322.2.3.4Westernblot法检测CASE对TGF-1介导的Smad7及PAI-1蛋白表达的影响.....................................................................................................................................322.2.3.5荧光实时定量RT-PCR法检测CASE对TGF-1诱导的PAI-1mRNA水平的影响......................

7、...........................................................................................................322.2.4瘢痕疙瘩成纤维细胞内MAPK通路对TGF-/Smad信号转导的调控作用...332.2.4.1细胞培养与分组................................................................................................332.2.4.2免疫沉淀与wester

8、nblot