丹参素对氧化低密度脂蛋白致血管平滑肌细胞增殖和凋亡的影响和机制

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1、Abstraetwereculturedassameastheabovefor3daysthenhandledasfollow：①5％FBSDMEM,(g)5％FBSDMEM+50ms／lox—LDL；③15％FBSDMEM+50mg／!ox-LDL+50ms／I：DSS；@)5％FBSDMEM+50ms／lox-LDL+100ms／LDSS．20hourslater,thecellsproliferationweredetectedbyMTTmethod．Theexperimentwasrepea

2、ted3times．Thecellswasharvestedin4boreof6-wellplateswith6x104cellseverybore．Thecellswereculturedassameastheabovefor3daysthenhandledassameastheabove．20hourslater,thecellsm—RNAofeachborewereextractedandtheexpressionofPDGF·-BBmRNAofeachborewasdetectedbyRT-

3、·PCRmethod．Theexperimentwasrepeated3times．6．Theeffectsofdifferentconcentrationofdanshensu(DSS)ontheapoptosisofVSMCinducedbyhighconcentrationOX-LDL：Thecellswereharvestedin4boreof6-wellplateswith6x104cellseveryhole，thecellswasculturedfor3daystheneachbore

4、washandledasfollow：①5％FBSDMEM；@)5％FBSDMEM+100rag／!OX—LDL；③5％FBSDMEM+100ms／!ox—LDL+50ms／LDSS；@5％FBSDMEM+lOOms／lox·LDL+100ms／LDSS．Themorphousofcellsofeachborewereobservedandapoptosisindexofeachborewasdetectedbyflowcytometry．Theexperimentwasrepeated3times

5、．，7．Statisticalanalysis：TheSPSSstatisticalpackage(version11．5)wasemployedtotestthedifferenceusingunivariateanalysisofS-N-Kandqtest．Results：1．TheprimarycultureandidentificationofratarterialVSMCs：TheVSMCswereculturedbyarepeatedlybloodvesselpatch-attachin

6、gmethod，largenumberofVSMCswereobtainedinashorttime．3dayslater，thecellsradiativelyanddiversifiedgrewaroundthebloodvesselpatchesthatwerewellattched．4to10dayslater，thecellsgrewinnumberandparallellyconverdedintofasciculationorradiation．Thespecificpeakorval

7、leyfeatureofVSMCswasabvious．Cellsgrewcondenselyandpassaged．Immunohistochemistrystain：myofilamentappearedincytopiasmwhichspreadparallellyandlongitudinallywithdensealea．PositiveVSMCs(browngranule)stainedbyirnmunohistochemistrywerefound．2．Thegrowthcharact

8、eristicsoftheprimaryratarterialsmoothmuscleVlAbstractcells：Thelocalcellsweresodensethat咖bepassegedby1：1or1：2．Severalhoursafterpassage，theadherenceofsomecellswereobserved．After3·4daysthecellsovergrowandcouldbepassegedby1：3or1：4．3．Theprep