mthfr基因多态性及环境因素与食管癌关系的研究

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1、(2)影响食管癌患病的环境因素主要有饮酒、热烫饮食、霉变食品摄入、空气污染状况、三餐定时、长期精神状态、遇事态度等。(3)MTHFRC677T变异及MTHFRA1298C变异与饮酒、热烫饮食、霉变食品摄入、遇事态度、长期精神状态、年龄间存在一定的交互作用。(4)食管癌患者体内部分微量元素铜、铁、锌等较正常人均有变化。(5)食管癌患者体内免疫状况发生变化，存在Thl／Th2漂移现象。关键词食管癌MTHFR环境因素交互作用病例对照研究3ASSOCIAnONRESEARCHBETWEENPOL'n订ORPHISMOFMTHFR,ENVIRONMENTALRI

2、SKFACTORS，ANDSUSCEPTIBILITYTOESOPHAGEALCANCERPostgraduate：WangQianSpeciality：EpidemiologyandHealthStatisticsDirectors：Prof．JingXue-anProf．LiDongAbstractobjectiveToexplorethepolymorphismof5，10-methylenetetrahydrofolatereductase(MTHFR)anditsassociation、砘n1susceptibilitytoesophagea

3、lcancer，aswellastheinteractionbetweentheenvironmentaldskfactorsandthepolymorphismofMTHFR．Toexplorethedifferenceofmicroelementcontentbetweenthecasesandthecontrols．Toexplorethechangesofthecytokine1h1andcytokineTh2betweenthecasesandthecontrols．’MethodPCR-RFLPtechnologywasusedtodete

4、rminethegenotypesofMTHFRC677TandMTHFRA1298C：AtomicabsorptionspectrometryWasusedtodetectthemicroelementcontent；EnzymelinkedimmunosorbentassaymethodwasusedtodetectIL··10andIL·-12；Allsubjectswereinvestigated、析msamequestionair．StatisticsanalysisWascarriedoutbySAS9．0andSPSS13．0statis

5、ticalpackagewithZ2-test、t-testandlogisticregressionanalysis．Result1．MTHFRC677Thavethreekindsofgenotypewhichwerewildhomozygote(CC)、mutationheterozygote(CT)、mutationhomozygote(邛．Frequenciesofthemwere38．2％、46．1％、15．7％incasegroup，and53．7％、33．3％、13．O％incontrolgrouprespectively(x2=5．1

6、456，P--0．0763)：AllelefrequenciesofTwere38．7％incasegroup，higherthan29．6％incontrolgroup(Z名3．8655，P--0．0493)；OR(95％CI)ofMTHFR677CTandTTgenotypeWas1．874(1．081—5．248)．2．MTHFRA1298Chavethreekindsofgenotypewhichwerewildhomozygote(AA)、mutationheterozygote(AC)、mutationhomozygote(CC)．Freq

7、uenciesofthemwere86．3％、12．7％、1．O％，incasegroup，and92．6％、6．5％、O．9％incontrolgrouprespectively4(，-2．3965，P--0．3017)；OR(95％CI)ofMTHFR677CTand1298AAgenotypewasl．912(1．013~3．607)；OR(95％C／)ofMTHFR677CTand1298ACgenotypeWaS5．687(1．112~29．086)．3．Analysisofmultiplelogisticregressionshowedth

8、atdrinkindex、likingtoeathotandharddiet，mildewfo