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1、中国肺癌杂志2009年7月第12卷第7期ChinJLungCancer,July2009,Vol.12,No.7·785··临床经验·肝素酶及Ki-67在非小细胞肺癌中的表达及临床意义宋作庆徐萧洪韦森陈军刘懿李昕赵洪林邱小明周乃康【摘要】背景与目的已有的研究表明,肝素酶与Ki-67抗原与肺癌的发生和转移有关,本研究通过检测非小细胞肺癌(Non-smallcelllungcancer,NSCLC)组织中肝素酶(heparanase,Hpa)和Ki-67的表达,以探讨他们的临床意义及相互关系。方法采用
2、免疫组化方法(S-ABC法和S-P法)检测70例NSCLC和20例同期良性病变肺组织中肝素酶和Ki-67的表达。结果肝素酶和Ki-67的阳性表达率分别为72.9%和85.7%,而正常肺组织中两者均无表达;肝素酶表达水平与患者TNM分期及淋巴结转移有关(P=0.044,P=0.001);Ki-67的表达水平与原发肿瘤大小、组织学类型、癌细胞分化程度、淋巴结转移及TNM分期有关(P<0.05);肝素酶和Ki-67的表达差异无统计学意义(P=0.323)。结论肝素酶和Ki-67参与了肺癌的发生和发展,并
3、与预后相关;而肝素酶和Ki-67的表达无相关性,两者可能通过不同的机制参与了肿瘤的发生和发展。【关键词】肺肿瘤;肝素酶;Ki-67;免疫组化【中图分类号】R734.2DOI:10.3779/j.issn.1009-3419.2009.07.008ExpressionandClinicalSignificanceofHeparanaseandKi-67inNon-smallCellLungCancer121111113ZuoqingSONG,XiaohongXU,SenWEI,JunCHEN,YiL
4、IU,XinLI,HonglinZHAO,XiaomingQIU,NaikangZHOU1DepartmentofLungCancerSurgery,TianjinLungCancerInsititute,TianjinMedicalUniversityGeneralHospital,Tianjin300052,23China;IntensiveCareUnite,TianjinMedicalUniversityGeneralHospital,Tianjin300052,China;Departm
5、entofThoracicSurgery,PLAGeneralHospital,Beijing100853,ChinaCorrespondingauthor:NaikangZHOU,E-mail:znklgp@sina.com【Abstract】BackgroundandobjectiveIthasbeenproventhatheparanaseandKi-67hadrelationshipwithoccur-renceandmetastasisinlungcancer.Theaimofthisw
6、orkwastoinvestigatetheexpressionsofheparanaseandKi-67andtoex-ploretheirclinicalsignificanceandmutualrelationsinnon-smallcelllungcancer(NSCLC).MethodsImmunohistochemicalmethodwasusedtodetecttheheparanaseandKi-67expressionintheroutinelyparaffin-embedded
7、sectionsofsurgicalspecimenof70caseswithNSCLCand20oneswithnormallungtissues.ResultsHeparanaseandKi-67werehighlyexpressedinlungcancertissues(72.9%and85.7%)whilenegativeinepitheliaofnormallungtissues.Theexpressionofheparanasewassignifi-cantlycorrelatedwi
8、thTNMstage(P=0.044)andlymphaticmetastasis(P=0.001).TheexpressionofKi-67wassignificantlycor-relatedwithtumorsize(P=0.03)andhistologytype(P=0.001)anddifferentiation(P=0.01)andlymphaticmetastasis(P=0.01)andTNMstage(P=0.043).Theexpressionofheparan