资源描述：
《姜科砂仁属植物DNA条形码序列的筛选.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、云南植物研究2010,32(5):393~400ActaBotanicaYunnanicaDOI:103724/SPJ1143201010070*姜科砂仁属植物DNA条形码序列的筛选1,21**杨振艳,张玲(1中国科学院西双版纳热带植物园,云南勐腊666303;2中国科学院研究生院,北京100049)摘要:砂仁属(Amomum)隶属于姜科,全属约150种,我国有39种。该属多种植物可作药物或香料,但目前砂仁属的分类还不清楚,准确鉴定物种有很大难度。本研究利用
2、DNAbarcoding技术,对砂仁属50种121个个体的matK、rbcLa、trnHpsbA序列及其不同组合进行比较,用TaxonDNA计算种间、种内barcodinggap,运用相似法的BLASTn计算条码的正确鉴定率,筛选适合砂仁属的条码片段。结果显示:所有条码的barcodinggap均不存在;matK的正确鉴定率高于trnHpsbA和rbcLa,联合片段的条码正确鉴定率高于单片段条码,三个片段联合条码的正确鉴定率最高。因此,推荐matK+rbcLa+trnHpsbA作为砂仁属物种鉴定的
3、候选条码。关键词:砂仁属;DNA条形码;matK;rbcLa;trnHpsbA;matK+trnHpsbA+rbcLa中图分类号:Q78,Q949文献标识码:A文章编号:02532700(2010)0539308ScreeningPotentialDNABarcodeRegionsinAmomum(Zingiberaceae)1,21**YANGZhenYan,ZHANGLing(1XishuangbannaTropicalBotanicalGarden,ChineseAc
4、ademyofScience,Mengla666303,China;2GraduateUniversityofChineseAcademyofSciences,Beijing100049,China)Abstract:Amomumcontains150species,and39ofthemarefoundinChina.ManyAmomumspeciesareusedintraditionalChinesemedicinesandfavoritefoodcondiment.Theintragenericclas
5、sificationofthisgenusislargelyunclear,andtheaccurateidentificationofspeciesisdifficult.DNAbarcodingisatechniquetoidentifyspeciesusingstandardizedDNAregion.TheCOIsequencehasbeensuccessfullyusedtoidentifyanimalspecies.Unfortunately,therearenouniversallyaccept
6、edbarcodesystemsforplants.Thisstudycomparedtheeachthreecandidateplastidregions(matK,rbcLa,trnHpsbA)andthecombineddata(matK+rbcLa,matK+trnHpsbA,rbcLa+trnHpsbA,matK+rbcLa+trnHpsbA)among121accessionsrepresenting50speciesofthegenus.Inordertoscreenoutthes
7、uitablebarcodeforidentifyingthespeciesofAmomum,TaxonDNAwasusedtoestimatethebarcodinggapbetweeninterspecificandintraspecificdistances,andBLASTnwasusedtocalculatetherateofcorrectidentification.TheresultsindicatedthattherewasnoDNAbarcodegapinthreeloc.iThecorre
8、ctidentificationofmatKwashigherthanthatoftrnHpsbAorrbcLa,thecombineddatahashighercorrectidentificationthanthatofsingleone,andthethreelocuscombineddatashowedthegreatestlevelofspeciesdiscrimina
