资源描述：
《健康成人与住院病人肠球菌致病基因及表型和耐药性的比较研究》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、健康成人与住院病人肠球菌致病基因及表型和耐药性的比较研究马立艳许淑珍（首都医科大学附属北京友谊医院，北京100050）【摘要】目的调查健康成人与住院病人分离肠球菌致病基因、致病表型和耐药性的携带情况，以了解肠球菌在医院感染中的意义。方法应用PCR检测肠球菌cylA、gelE、esp、ace、agg和efaA等6种致病基因，并用斑点杂交和基因测序确证。应用β溶血试验、明胶溶解和生物膜形成检测3种致病表型。应用琼脂稀释法检测肠球菌对高浓度庆大霉素、高浓度链霉素、万古霉素和氨苄西林的耐药性。结果健康成人和住院病人粪肠球菌中均检测到6种致病基因，前者检出率7.4～40.7％，后
2、者检出率29.0～80.6％；屎肠球菌健康成人分离菌株检测出3种致病基因，检出率5.6～16.7％；住院病人分离菌株检测出4种致病基因，检出率21.1～57.9％。健康成人和住院病人肠球菌β溶血、明胶溶解和生物膜形成的检出率分别为26.7％，54.0％；13.3％，40.0％；97.8％，90.0％。健康成人和住院病人屎肠球菌耐药率分别为22.2～94.4％，68.4～100.0％；粪肠球菌分别为3.7～33.3％，29.0～64.5％。结论健康成人分离株较住院病人分离株的致病性弱，屎肠球菌比粪肠球菌的耐药性高。【关键词】肠球菌属；致病性；耐药；基因Studyonvir
3、ulencedeterminants,phenotypesanddrugresistanceofenterococcusfrominpatientsandhealthyadultsMALi-yan,XUShu-zhen(BeijingFriendshipHospital,CapitalUniversityofMedicalSciences,Beijing100050,China)【Abstract】ObjectiveTocomparetheincidenceofsixvirulencedeterminants,threephenotypesanddrugresistan
4、ceinenterococciisolatedfrominpatientsandhealthyadults.Tofindoutthesignificanceofenterococcusinnosocomialinfection.MethodsPCRwasusedtodetectthesixvirulencedeterminants,includingcylA,gelE,esp,ace,aggandefaA.Sequencinganddotblotweretakenasconfirmationtests.Andthethreephenotypes,includingβ-h
5、emolysis,gelatinhydrolysisandbiofilmformationweredeterminedonagarplatecontaining7%rabbitblood,Todd-Hewittagarplatesupplementedwith3%gelatinand96wellpolystyreneplates,respectively.Antibioticsusceptibilitytestwasperformedbyagardilutionmethod.ResultsThedetectionratesofthesixvirulencedetermi
6、nantsofEnterococcusfaecaliswere:7.4-40.7%inhealthyadults,29.0-80.6%ininpatients.InE.faeciumstrains,thenumberofdetectedgenesandthedetectionrateswere:3and5.6-16.7%inhealthyadults,4and21.1-57.9%ininpatients,respectively.Theβ-hemolysis,gelatinhydrolysisandbiofilmformationofisolatedstrainsfro
7、mhealthyadultsandinpatientswere26.7％,54.0％;13.3％,40.0％;97.8％,90.0％,respectively.E.faeciumhaveahigherresistantpercentagetoAmp,highlevelGmandSmthandoE.faecalis.ConclusionsStrainsisolatedfromhealthyadultsaremuchlesspathogeniticthanthatfrominpatients.DrugresistanceofE.faecium
