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1、#临床药学#$HPLC法测定肾移植患者血浆中霉酚酸的浓度叶丽卡,谢志红,王若伦,许欣,张伟婷(广州医学院第二附属医院,广州市510260)中图分类号R96911文献标识码A文章编号1001-0408(2008)17-1306-03摘要目的:建立以高效液相色谱法测定霉酚酸血药浓度的方法,并研究其在中国肾移植患者体内的多剂量药动学特征。方法:血-1-1浆采用乙腈蛋白沉淀后进样。其中,色谱柱为DiamonsilC18,流动相为乙腈-10mmol#LKH2PO4(5B6),流速为111mL#min,检测波长为254nm,柱温为40e。此方法应用于测定12例肾移植患者的多剂量药动学。结
2、果:色谱中霉酚酸与内标分离良好,血浆-1-1中的内源性杂质无干扰。霉酚酸检测浓度在0138~59100Lg#mL范围内线性关系良好,最低检测限为0138Lg#mL;回收率为89132%~97163%,日内、日间RSD均<8100%。药动学结果显示患者服用霉酚酸酯后,霉酚酸的药动学个体差异较大,与文献报道基本一致。结论:本方法结果准确,操作简便,适合于霉酚酸的药动学研究。关键词霉酚酸;高效液相色谱法;肾移植患者;血药浓度;多剂量;药动学DeterminationofMycophenolicAcidinKidneyTransplantationPatientsbyHPLCYELi
3、-ka,XIEZhi-hong,WANGRuo-lun,XUXin,ZHANGWei-ting(TheSecondAffiliatedHospitalofGuangzhouMedicalCollege,Guangzhou510260,China)ABSTRACTOBJECTIVE:TodeterminetheplasmamycophenolicacidconcentrationbyHPLC,andstudythemultidosespharmacokineticscharacterofmycophenolicacidinChinesekidneytransplantation
4、patients1METHODS:Thesampleswereprecipitatedwithacetonitrilebeforeinjection1DiamonsilC18columnwasused1Themobilephaseconsistedofacetonitrile-10-1-1mmol#LKH2PO4(5B6)ataflowrateof111mL#min1Thedetectionwavelengthwas254nmandthecolumntemper-aturewas40e1Thismethodwasusedtodeterminethemultidosesphar
5、macokineticsin12kidneytransplantationpatients1RESULTS:MPAwaswell-separatedfrominternalstandardinchromatography,andendogenousforeignsubstanceinplasma-1hadnointerferenceonthedetermination1ThelinerrangeforMPAwas0138~59100Lg#mL,andthelowestdetectable-1concentrationofMPAwas0138Lg#mL1Therecoveryr
6、atestoodat89132%~97163%;Bothintra-dayandinter-dayRSDwerelessthan8100%1SignificantindividualdifferencewasnotedamongthepatientstreatedwithMMFinpharma-cokineticresults,whichwasinlinewiththeliterature1CONCLUSION:Thismethodisaccurateandsimpleandapplicableforthepharmacokineticsstudyofmycophenolic
7、acid1KEYWORDSMycophenolicacid;HPLC;Kidneytransplantationpatients;Plasmadrugconcentration;Multidoses;Phar-macokinetics霉酚酸酯(Mycophenolatemofetil,MMF)是一种常用量MMF(500mg,bid)后的药动学特征,但国内不少肾移植术免疫抑制药物,于1998年在我国上市,被广泛应用于防治器官后患者长期服用MMF(500mg,bid)。本研究拟采用内标法,移植后的免疫排斥反
