圆弧青霉pg37碱性脂肪酶的发酵生产、分离纯化及基因克隆和表达

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1、复旦大学博士学位论文圆弧青霉PG37碱性脂肪酶的发酵生产、分离纯化及基因克隆和表达姓名：邬敏辰申请学位级别：博士专业：生物学指导教师：孙崇荣2000.9.1缩写词表——————————————————————————————————————————————————————————————一一AbbreviationswithChineseandEnglishInterpretation-缉呈墓室鲤猛AbA260ACNAgAmpATPbisbpBSAcAMPcDNADaDTTDNaseDNADEAEDEPCoNS—CldsEDTAAntibodyAbsorbancea

2、t260nmAcetonitrileAntigenAmpicUliuAdenosine5'-triphophateN．N’·methylene—bisacrylamideBasepairBovineserumalbuminAdenosine3’，5’一cyclic-monophosphateComplementarydeoxyr．bonucleicacidDaltonDithiothreitolDeoxyribonucleaseDeoxyribonucleicacidDiethylaminoethylDiethylpyrocarbonateDansyichlorid

3、eDoublestrandedEthylenediaminetetraaceticacid1主塞鲤猛抗体260nm处吸光度乙膪抗原氨苄青霉素腺苷三磷酸N’N’．亚甲双丙烯酰胺碱基对牛血清白蛋白环腺背酸互补DNA道尔i顷二硫苏糖醇脱氧核糖核酸酶脱氧核糖核酸二乙基氨基乙基焦碳酸二乙酯丹磺酰氯双链乙二胺四乙酸⋯一竺竺望查～——————————————————————————一ECESIMSGSTHRPoHPLCIPTGⅡCF19GKmLASLipILipIILipHImRNAMDGLmdlCMMLVMoPSNTG0RFoligo(dT)pIPEGPG37PB227Enzy

4、meCommisionElectrosprayionizationmassspectrometryGlutathioneS-transferaseHorseradishperoxidaseHigh—performanceliquidchromatographyIsopropyl一1一thio—B—D—GalactosideIsoelectricfocusingImmunoglobulinGMichaelisconstantLinearalkylbenzenesulfonateAlkalinelipaseIAlkalinelipaseIIAlkalinelipaseI

5、IIMessengerribonucleicacidMono—anddiacylglycerollipaseGeneencodingMDGLfromPG37Moloneymurineleukemiavirus3一(N·morpholino)propanesulphonieacidN—methyle—N—nitro—N-nitrosoguanidilleOpenreadingframeOligodeoxythymidylicacidlsoelectricpointPolyethyleneglycolPenicilliumcyclopinmPG37Penicillium

6、cyclopiumPB2272酶学委员会电喷雾质谱谷胱苷肤s．转移酶辣根过氧化物酶高效液相色谱异丙基．硫代一D—D一半乳糖苷等电聚焦免疫球蛋白G米氏常数直链烷基苯磺酸按碱性脂肪酶I碱性脂肪酶Ⅱ碱性脂肪酶11I信使核糖核酸单双脂酰廿油脂肪酶PG37MDGL基因鼠白血病病毒3一(N一吗啉代)丙磺酸亚硝基胍开放阅读框架寡聚脱氧胸苷酸等电点聚乙二醇圆弧青霉PG37菌株圆弧青霉PB227菌株缩写词表poly(A)PAGEPBSPVArRNART—PCRPCRRERP—HPLCRNaseSDSSSTAEtspTEMEDPolyadenylicacidPolyacrylamide

7、gelelectrophoresisPhosphate—bufferedsalinePolyvinylalcoholRibosomeribonucleieacidReversetransci’iptionPCRPolymerasechainreactionRestrictionendOnucleaseReversedphaseHPLCRibonucleaseSodiumdodecylsulfateSinglestrandedTris．acetatebufrerTranscriptionstartpointN，N，N’，N’一tetramethylethylene