伽玛刀照射兔视神经后电生理与病理组织学改变的实验-研究

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1、天津医科大学硕士学位论文msand(2．32士O．37)gV．Comparewithnormalgroup，8GygroupP100latencyandamplitudeateachtimepointswasnosignificantdifference(P>0．05)．10GygroupP100latencyextendatthe5thmonthandamplitudedeclineatthe6thmonthafterirradiation，comparedwiththenormalgroup，thedifferencesweresign

2、ificant(P<0．05)．12Gygroupatthe2ndweekthelatencywassignificantlyprolongedandatthe1stmonthamplitudesignificantlydecline，comparedwiththenormalgroupweresignificantlydifferent(P<0．01)．15Gygroupatthe1stweekafterirradiationP100latencywasprolongedandatthe1stweekamplitudesignifican

3、tlydecline，comparedwiththenormalgroup(P<0．01)．OvertimetheelectrophysiologicalchangeswasprogressivedelayinP100latencyandprogressivereducedinamplitude．2．PathologicalobservationsresultsUnderlightmicroscopy：ThenormalgrouprabbitsopticnerveHEstaining，myelinstainingandNFimmunohis

4、tochemicalstainingtodrawpositivestainingresults．HEstaining8Gygroupateachtimepointappearperivascularedemaandexudation，comparewiththenormalgroup．Inthe10Gygroupatthe3rdmonthappeartheperivascularedemaandexudation，vascularlumennarrowing，uneventhickeningofthevesselwall，thesechan

5、gesatthe6thmonthgroupmoreobviousthanthe3rdmonth．12Gygroupand15Gygroupalsoshowthesamevascularchangesjustlike10Gygroup．Myelinstainingatthe3rdandthe6thmonthofeachdosegroupshowednosignificantpathologicalchangescomparedwiththenormalgroupexcepttheperivascularchanges．NFstainingat

6、the3rdandthe6thmonthofeachdosegroupshowsignifcantpathologicalchanges，comparedwiththenormalgroupexcepttheperivascularchanges．Underelectronmicroscopy：Thenormalgroupopticnervemyelinstructureiscomplete，arrangedorderly，intheaxoplasmmicrofilamentandmicrotubuledistributionuniform

7、，mitochondfialstructureisnormal．8Gygroupunderelectronmicroscopyatthe3rdmonthshowswellingofthemitochondfia，comparewiththenormalgroup，butatthe6thmonththechangesreturnedtobenormal．10Gygroupatthe3rdmonthshowobviousmitochondrialswelling，rupture，microfilamentsandmicrotubulesspar

8、sedensity，demyelinationisrare，comparedwiththe3rdmonth，the6thmonthshowsignificantmyelindis