壮筋续骨汤对骨折大鼠血清bfgf和igf-1含量影响和意义

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1、TheEffectofZhuangJinXuGuDecoctioninPromotingtheExpressionofCGRPandIGF一1inRatsABSTRACTobjective：ToresearchtheeffectofZhuangJinXuGuDecoctioninpromotingbFGFandIGF-1expressioninfemoralfracturecallus，andthentodiscussthefracturehealingfunctionofZhuangJinXuGuDecoction．Methods：To

2、talofseventy-twoadultandhealthymaleWistarrats．whichweremadefracturemodelwithelectricsawthatwerecutthroughthemiddlepointoffemur．ThemodelgroupwastreatedbyinfusingZhuangJinXuGuDecoctionthroughstomach．ThentoObservethebodyactivitiesindifferentdays，thefracturehealingsituationwa

3、sobservedthroughtthewayofX-rayfilm，inthelastthepathologicalchangewasdetectedbyHematoxylin-Eosinstaining(HE)．TheserulTllevelsofbFGFandIGF-1weregetbythewayofenzyme-linkimmunobsorbantassay(ELISA)．Results：localswellingandrestrictedmovementafterFemoralfi'actures．7claysaftertre

4、atment，localswellingdisappeared，14days，fibrouscallusformation,21days，fracturelinesblurred,28daysfracturelinedisappearedfromthetestofXray．Inthemodelgroup，theexpressionsofbFGFandIGF-1Washigherthantheratsinsham—operatedgroup(P<0．05)．TheserumlevelsofbFGFandIGF一1intreatmentgro

5、upwerehigherthanthoseinmodelgroup(P<0．05)．21daysaftertreatment,theexpressionofserumbFGFandIGF—IWastoaspike．Conclusion：ZhuangJinXuGuDecoctionhavetheeffecttopromotingfemoralfracturecallusofrats,whichmightplayapartinincreasingtheexpressionsofbFGFandIGF一1Postgraduatestudent：P

6、anYue—xingDirectedbyProfessor：GuoYun-liangKeywords：ZhuangJinXuGuDecoction；Fracture；bFGF；IGF·1；Wistarrats目录引言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯“l材料和方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯21材料⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”22方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

7、⋯⋯⋯⋯⋯⋯⋯⋯．．33评价指标⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯“44统计学处理⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯5结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”61x线观察⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”62大体观察⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”63病理变化⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”74ELISA检测⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

8、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯8讨论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”9结论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯