江门病毒性腹泻患者中星状病毒感染的分子流行病学研究

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1、ABSTRACT&Outpatientdepartmentinstead’collectingfecalspecimensfromthepatientswithacutesporadicdiarrheaduringthepeakseasonbetweenSep2006andJan2007．2．ThestoolswerefirstscreenedforRotavirus(RV)andNorwalk—likevirus(NV)withRotavimsGroupADiagnosticKitandreverse-transeriptionpolyrnerasechainreaction

2、(RT-PCR)respectively，followedbyRealTimeTaqManPCRdetectingHAstV．HAsWpositivestoolsfromTaqManPCRwereconformedbyRT-nestedPCR,withsubsequentampliconspurifiedandsequencedlastly．NucleotidesequenceswerealignedwithhomologoussequencesinGeneBanktodeterminethegenotypeswithBlastnSoftware，followedbyPhylo

3、genetictreeemployedforstudyingthevarietiesanddiversityofHAstVgenotypes．3．ToasSeSthemediealimportanceofHAstVinfection，theel赫ealcharacteristiesofchildreninfectedwithHAsWwerecomparedwiththosefoundinpatientsinfectedwithRV．Anda1：1case—controlstudyWasperformedwiththeobjectiveofidentifyingtheriskfa

4、ctors硒fsporadicHAstVinfection．Returnedquestionnaireswerecodedanddouble—enteredintoEpiData2．1a．QualitativedatastatisticalanalysisWasoperatedwithChi—squaretest，andquantitativedataWasinsteademployedwith2samplet-testforindependentsamplesornonparametrietestbySPSSI3．0，basedon仪wasvaluedas0．05．Allte

5、stsweretwo-tailedandconsideredsignificantwhentheP-valuewas<0．05．A1：1conditionallogisticregressionmodelwasusedtostudytheindependenteffectsofriskfactorswithanassociationintheunivariateanalyseswithaP—value<0．05．SelectionofvariablesinthemodelWasstepwisemanually,basedonthelog-likelihoodratio．Resu

6、lts：1．ThetotalprevalencerateofchildrencaseswithHAstVinfectioninJiangmenregionwasS．5％(40／470)byTaqManRealTimePCR,duringtheyearperiodfromSep．2005toAug．2006，withit’SpeakprevalencerangingfromSeptembertoDecember．HAstV-associateddiarrheahasasoloinfectionrateof27．5％，comparedtomixedonesof72．5％，which

7、included67．5％RVand5％NV．ii硕士学位论文AstothosewholessthanfiveyearsoldgroupsstrickenbyHAstVinfection，Nosignificantstatisticdifferencewerefoundamongages，aswellasbetweenmalesandfemales．EighteenoutoffortystrainsofHAstVinfectionscreenedinsystematicsamplingway