苏州地区5岁以下儿童轮状病毒性腹泻的分子流行病学研究

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1、：１０３１２分类号：Ｒ６单位代码：２０１５１６４４密级：学号硕士学位论文苏州地区５岁以下儿童轮状病毒性腹泻的题目：分子流行病学研究研究生：徐承指导教师：学科专业：临床检验诊断学二学院名称：南京医科大学第临床医学院二一八年五月完成时间：〇分类号：R6单位代码：10312密级：学号：20151644硕士学位论文苏州地区５岁以下儿童轮状病毒性腹泻的题目：分子流行病学研究研究生：徐承指导教师：朱叶飞学科专业：临床检验诊断学学院名称：南京医科大学第二临床医学院完成时间：二〇一八年

2、五月南京医科大学硕士学位论文缩略词表英文缩写英文全称中文全称RVRotavirus轮状病毒ReversetranscriptionpolymeraseRT-PCR反转录聚合酶链式反应chainreactionASantigenicsite抗原位点dsRNADouble-strandedribonucleicacid双链核糖核酸VPViralprotein结构蛋白NSPNonstructuralprotein非结构蛋白TBETrisboricacidEDTATris-硼酸电泳缓冲液DEPCDiethylpyro

3、carbonate焦碳酸二乙酯DNADeoxynucleicacid脱氧核糖核酸VRVariableregion可变区GMMonosialotetrahexosylganglioside神经节苷脂HBGAHisto-bloodgroupantigen血型组织抗原HSP-70Heatshockproteins70热休克蛋白70DLPDoublelayeredparticle双层蛋白颗粒ECEnterochromaffincell肠嗜铬细胞5-HT5-hydroxytryptamine5-羟色胺南京医科大学硕士学

4、位论文目录中文摘要························································································1Abstract··························································································4前言··························································

5、····································8第一部分苏州地区5岁以下儿童轮状病毒腹泻的流行病学特征·················11材料与方法···············································································11结果······················································································

6、··18讨论························································································23小结························································································26第二部分2010~2016年流行株G9P[8]的全基因组测序分析·······················27材料与方法·······

7、········································································27结果························································································33讨论························································································4

8、9小结························································································53参考文献························································································54文献综述··········