vn对高糖培养下huvec作用的初步探讨

《vn对高糖培养下huvec作用的初步探讨》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、objectiveThePreliminaryResearchontheRoleofVitronectininHUVECTrainedByHighGlucoseAbstractThisstudywastoobservetheexpressionofvitronectin,thesituationofcytoskeletonremodeling，cellmigrationandbloodvesselsforminginHUVECtrainedby11ighglucose．Andtosurveytheinfluenceofvitronectinoncytoskeleton

2、remodeling，cellmigrationandbloodvesselsform崦inHUVEC．MethodsThefirstpart：HUVECwereculturedrespectivelybynormalandhighglucose．Theexperimentsweredividedintogroupl(normalglucosegroup：HUVECweretrainedbyDMEMmediumwhichcontained25mmol／Lglucose)andgroup2(highglucosegroup：HUVECweretrainedbyDMEMm

3、ediumwhichcontained50mmol／Lglucose)．Vitronectinproteinexpressionweremeasuredbywesternblot，andthemicrofilamentcytoskeletonofHUVECwereobservedbyimmunofluorescencecytochemicalstainingcombined谢tllfluorescencemicroscope．Atlast，evaluatedcellmigrationabilitywithscratchwoundassayandobservedbloo

4、dvesselsformingabilitywithmatrigelassay．ThesedatawereanalysedbyIndependentTtestsample．Thesecondpart：DisturbedtheexpressionofvitronectininhighglucosecasemakeuseofRNAinterferencetechnology．Theexperimentsweredividedintogroup2olighglucosegroup：HUVECweretrainedbyDMEMmediumwhichcontained50mmo

5、l／Lglucose)，group3(negativeinterferencegroup：HUVECwereinterferencedbycontrolSiRNAinadvance，andthenweretrainedbyDMEMmediumwhichcontained50mmol／Lglucose)andgroup4(positiveinterferencegroup：HUVECwereinterferencedbyVNSiRNAinadvance，andthenweretrainedbyDMEMmediumwhichcontains50mmol／Lglucose)

6、．Repeattheexperiments，incluingwesternbltot，immunofluorescencecytochemicalstaining，scratchwoundassayandmatrigelassay．Alldataswereanalysedbytestofhomogeneityofvariancesfirst,thenwenconductedFtest．IfthedifferenceswereobviousinFtest，thesedatawereanalysedbyLSDofOnewayANOVAfLLrther．Resuits．Th

7、efirstpart：Vitronectinproteinexpressioningroup1andgroup2wererespectively(1．098+0．085)(1．091士0．068)at0hours，andthedifferenceswerenotobvious(tl'2=0．111，P12>0．05)；After24hours，vitronectinproteinexpressioningroup1andgroup2wererespectively(0．8934-0．119)(1．2954-0．080)；Andafter48hours