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ID:33332004
大小:1.45 MB
页数:41页
时间:2019-02-24
《vn对高糖培养下huvec作用的初步探讨》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、objectiveThePreliminaryResearchontheRoleofVitronectininHUVECTrainedByHighGlucoseAbstractThisstudywastoobservetheexpressionofvitronectin,thesituationofcytoskeletonremodeling,cellmigrationandbloodvesselsforminginHUVECtrainedby11ighglucose.Andtosurveytheinfluenceofvitronectinoncytoskeleton
2、remodeling,cellmigrationandbloodvesselsform崦inHUVEC.MethodsThefirstpart:HUVECwereculturedrespectivelybynormalandhighglucose.Theexperimentsweredividedintogroupl(normalglucosegroup:HUVECweretrainedbyDMEMmediumwhichcontained25mmol/Lglucose)andgroup2(highglucosegroup:HUVECweretrainedbyDMEMm
3、ediumwhichcontained50mmol/Lglucose).Vitronectinproteinexpressionweremeasuredbywesternblot,andthemicrofilamentcytoskeletonofHUVECwereobservedbyimmunofluorescencecytochemicalstainingcombined谢tllfluorescencemicroscope.Atlast,evaluatedcellmigrationabilitywithscratchwoundassayandobservedbloo
4、dvesselsformingabilitywithmatrigelassay.ThesedatawereanalysedbyIndependentTtestsample.Thesecondpart:DisturbedtheexpressionofvitronectininhighglucosecasemakeuseofRNAinterferencetechnology.Theexperimentsweredividedintogroup2olighglucosegroup:HUVECweretrainedbyDMEMmediumwhichcontained50mmo
5、l/Lglucose),group3(negativeinterferencegroup:HUVECwereinterferencedbycontrolSiRNAinadvance,andthenweretrainedbyDMEMmediumwhichcontained50mmol/Lglucose)andgroup4(positiveinterferencegroup:HUVECwereinterferencedbyVNSiRNAinadvance,andthenweretrainedbyDMEMmediumwhichcontains50mmol/Lglucose)
6、.Repeattheexperiments,incluingwesternbltot,immunofluorescencecytochemicalstaining,scratchwoundassayandmatrigelassay.Alldataswereanalysedbytestofhomogeneityofvariancesfirst,thenwenconductedFtest.IfthedifferenceswereobviousinFtest,thesedatawereanalysedbyLSDofOnewayANOVAfLLrther.Resuits.Th
7、efirstpart:Vitronectinproteinexpressioningroup1andgroup2wererespectively(1.098+0.085)(1.091士0.068)at0hours,andthedifferenceswerenotobvious(tl'2=0.111,P12>0.05);After24hours,vitronectinproteinexpressioningroup1andgroup2wererespectively(0.8934-0.119)(1.2954-0.080);Andafter48hours
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