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ID:33235672
大小:2.90 MB
页数:44页
时间:2019-02-22
《静脉注射多巴胺致组织损伤及其防护措施的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、遵义医学院硕士学位论文静脉注射多巴胺致组织损伤及其防护措施的实验研究姓名:陶明申请学位级别:硕士专业:护理学指导教师:江智霞201205遵义医学院硕士学位论文静脉注射多巴胺致组织损伤及其防护措施的实验研究中文摘要目的:探讨静脉注射多巴胺对组织的损伤情况及防护措施。方法:(1)将实验动物随机分为阴性对照组:直接静脉滴注生理盐水;阳性对照组:静脉输入20mg多巴胺/lOOml生理盐水;实验组:采用放血法制作低血压模型成功后,在低血压状态下静脉输入20mg多巴胺/100M生理盐水。分别于用药后0.5h、1h、2h、4h采集血液标本测定
2、炎细胞因子浓度以观察全身炎症反应情况,同时以静脉穿刺点为中心取长2.5cm、宽1.5cm包含注射血管的组织做病理学观察局部损伤情况。(2)方法:将实验动物随机分为京万红组、无处置组、湿润烧伤膏组、硫酸镁组,均连续3d静脉输入高浓度多巴胺,同时于输液部位皮肤分别予以外用京万红软膏、不处置、外用湿润烧伤膏、外用33%硫酸镁溶液。输液完毕,先对局部静脉炎情况进行判断,然后分别取输液血管进行组织学观察、采集血液标本作炎性细胞因子浓度测定。结果:(1)①炎细胞因子组间比较:三组炎细胞因子在2h、4h时间点比较有差异(P<0.05),低血压
3、组高于两对照组;组内比较:正常血压组、实验组各时间点比较总体有差异(氏0.05);②病理学改变组间比较:三组总阳性率有差异(P4、的实验研究IntravenousdopaminetorabbittissuedamageandprotectivemeasuresofexperimentsAbstractObjective:Toprobeintothedamnificationoftissueandmeasureofpreventionwithintravenousdopamine.Methods:(1)Theexperimentalrabbitswererandomlydividedintongative,positiveandexperimentalgro5、upswhichintravenousinfusionofsaline,dopaminesaline(20mg/100m1)withnormalbloodpressureanddopaminesaline(20mg/lOOml)afterphlebotomyproducedhypotensionmodelsuccessfully,respectively.Bloodsamplesforthedeterminationoftheconcentrationofinflammatorycytokinesinordertoobserve6、thesystemicinflammatoryresponseafertreatmentO.5h,lh,2hand4h,respectively.Meanwhile,Wetaketheinjectedvasaltissuewith2.5cmwidthand1.5cmlengthtoobserveitspathologicalchangesinthecenterofthedotofvenipuncture.(2)ExperimentalrabbitswereequallydividedintoJingwanhonggroup,no7、disposalgroup,theMEBOgroupandmagnesiumsulfategroup.Therabbitsofallgroupsweretransfusedwithhi曲doseofdopamineundergeneralanesthesiafor3days.Thesametime,attheinfusionsitewereusedonjingwanhongointment,noothertreatment,MEBOand33%magnesiumsulfate.Aftertheinfusing,bloodvess8、elsamplesintheinfusingareawerecollectedtodohistologicalobservationandbloodsamplesweretakenforthedeterminationofinflammationfactorsR
4、的实验研究IntravenousdopaminetorabbittissuedamageandprotectivemeasuresofexperimentsAbstractObjective:Toprobeintothedamnificationoftissueandmeasureofpreventionwithintravenousdopamine.Methods:(1)Theexperimentalrabbitswererandomlydividedintongative,positiveandexperimentalgro
5、upswhichintravenousinfusionofsaline,dopaminesaline(20mg/100m1)withnormalbloodpressureanddopaminesaline(20mg/lOOml)afterphlebotomyproducedhypotensionmodelsuccessfully,respectively.Bloodsamplesforthedeterminationoftheconcentrationofinflammatorycytokinesinordertoobserve
6、thesystemicinflammatoryresponseafertreatmentO.5h,lh,2hand4h,respectively.Meanwhile,Wetaketheinjectedvasaltissuewith2.5cmwidthand1.5cmlengthtoobserveitspathologicalchangesinthecenterofthedotofvenipuncture.(2)ExperimentalrabbitswereequallydividedintoJingwanhonggroup,no
7、disposalgroup,theMEBOgroupandmagnesiumsulfategroup.Therabbitsofallgroupsweretransfusedwithhi曲doseofdopamineundergeneralanesthesiafor3days.Thesametime,attheinfusionsitewereusedonjingwanhongointment,noothertreatment,MEBOand33%magnesiumsulfate.Aftertheinfusing,bloodvess
8、elsamplesintheinfusingareawerecollectedtodohistologicalobservationandbloodsamplesweretakenforthedeterminationofinflammationfactorsR
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