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ID:33159431
大小:5.46 MB
页数:41页
时间:2019-02-21
《eryag激光与车针备洞对兔牙髓hsp70表达影响的对比研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、南昌大学硕士学位论文Er:YAG激光与车针备洞对兔牙髓HSP70表达影响的对比研究姓名:陈鹏申请学位级别:硕士专业:口腔临床医学指导教师:汪建中201205摘要illllllllllllllllllllllll
2、lllllllIY2157731摘要目的:分别采用Er:YAG激光与车针对兔牙行窝洞预备,通过免疫组化染色检测兔的牙髓细胞中HSP70的表达情况,比较两种处理对牙髓刺激的程度,并观察其随时间的变化情况。方法:将32只雄兔随机分为2组,即激光组(Er:YAG激光备洞)和车针组(车针备洞),每组16只。分别使用Er:YAG激光、车针对
3、雄兔下颌右侧中切牙进行窝洞预备,并在备洞后的第24h、3d、7d和14d将每组雄兔随机处死4只,制备牙髓石蜡切片进行免疫组化染色。通过测量平均光密度值(MeanOpticalDensity,MOD)来检测HSP70的表达情况,所有的数据使用SPSSl7.0进行统计分析。结果:1、两个处理组对HSP70表达的MOD值的影响存在显著差异(P<0.05);在不同时间点MOD值亦明显不同(P<0.05);且处理因素和时间因素存在交互作用(P4、14d时两者差异不明显(P>0.05)。3、在车针处理因素下各时间点组间的两两比较中,仅24h与7d对比组的MOD值差异较小(P>0.05);而其它各对比组的MOD值差异显著(P<0.05)。4、在激光处理因素下各时间点组间的两两比较中,仅7d与14d对比组的MOD值差异较小(P>0.05),而其它各对比组的MOD值差异显著(P<0.05)。结论:1、Er:YAG激光备洞所致牙髓组织病理学改变及HSP70的表达变化小于车针备洞;2、Er:YAG激光备洞所致牙髓组织病理学改变及HSP70的表达变化恢复速度要快于车针各洞。关键词:Er:YAG5、激光;备洞;HSP7011AbstractABSTRACTObjective:Cavitiespreparationweremadeonrabbitteethusingtwodifferenttools,whichwereEr:YAGlaserandhighspeeddrill.Aftercavitypreparationbytwokindsofstimulus,heatshockprotein70(HSP70)expressionbyimmunohistochemicalstainingwereobservedtoevaluatethe6、differenteffectsondentalpulp,andthechangingovertime.Method:32malerabbitswereusedinthisstudy,dividedinto2groupsrandomly,whichwerelasergroup(cavitieswerepreparedusingEr:YAGlaser)anddrillgroup(cavitieswerepreparedusinghighspeeddrill),16rabbitsineachgroup.Thecentralincisorsof7、rightmandibularwerechosentopreparecavities,afterthat,4rabbitswererandomlykilledon1,3,7,14drespectivelyforeachgrouptomakepulpparaffinsectionsforimmunohistochemicalstudy.HSP70expressionwereexaminedbymeasuringMeanOpticalDensityvalues(MOD),allthepresentdatawereanalysedbySPSS18、7.0software.Results:1.TheMODvaluesofHSP70expressioninthehighspeeddrillgroupwassignificantlydifferentwiththeEr:YAGlasergroup(P<0.05):theMODvaluesineachtimepointweremarkedlydifferent(P<0.05);moreover,thetreatmentfactorandthetimefactorhadinteractioneffects.2.Onthe1st,3rdand79、thdayaftercavitiespreparation,theMODvaluesofHSP70expressioninthehighspeeddrillgroupweresignifica
4、14d时两者差异不明显(P>0.05)。3、在车针处理因素下各时间点组间的两两比较中,仅24h与7d对比组的MOD值差异较小(P>0.05);而其它各对比组的MOD值差异显著(P<0.05)。4、在激光处理因素下各时间点组间的两两比较中,仅7d与14d对比组的MOD值差异较小(P>0.05),而其它各对比组的MOD值差异显著(P<0.05)。结论:1、Er:YAG激光备洞所致牙髓组织病理学改变及HSP70的表达变化小于车针备洞;2、Er:YAG激光备洞所致牙髓组织病理学改变及HSP70的表达变化恢复速度要快于车针各洞。关键词:Er:YAG
5、激光;备洞;HSP7011AbstractABSTRACTObjective:Cavitiespreparationweremadeonrabbitteethusingtwodifferenttools,whichwereEr:YAGlaserandhighspeeddrill.Aftercavitypreparationbytwokindsofstimulus,heatshockprotein70(HSP70)expressionbyimmunohistochemicalstainingwereobservedtoevaluatethe
6、differenteffectsondentalpulp,andthechangingovertime.Method:32malerabbitswereusedinthisstudy,dividedinto2groupsrandomly,whichwerelasergroup(cavitieswerepreparedusingEr:YAGlaser)anddrillgroup(cavitieswerepreparedusinghighspeeddrill),16rabbitsineachgroup.Thecentralincisorsof
7、rightmandibularwerechosentopreparecavities,afterthat,4rabbitswererandomlykilledon1,3,7,14drespectivelyforeachgrouptomakepulpparaffinsectionsforimmunohistochemicalstudy.HSP70expressionwereexaminedbymeasuringMeanOpticalDensityvalues(MOD),allthepresentdatawereanalysedbySPSS1
8、7.0software.Results:1.TheMODvaluesofHSP70expressioninthehighspeeddrillgroupwassignificantlydifferentwiththeEr:YAGlasergroup(P<0.05):theMODvaluesineachtimepointweremarkedlydifferent(P<0.05);moreover,thetreatmentfactorandthetimefactorhadinteractioneffects.2.Onthe1st,3rdand7
9、thdayaftercavitiespreparation,theMODvaluesofHSP70expressioninthehighspeeddrillgroupweresignifica
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