力竭运动与钝挫伤对大鼠骨骼肌卫星细胞与肝细胞生长因子的影响

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1、叶雷雷：力竭运动及钝挫伤对人鼠骨日hi口t肌比／Zj3二!删H岜以盯"细胞生K冈子的影响111ABSTRACTObjective：Thepurposeofthispaperwastoinvestigatedeffectsofone．timeexhaustiveexerciseandcontusiononskeletalmusclesatellitecellsandhepatocytegrowthfactor(HGF)inrat．Methods：24malewistarratsof6一week．oldwererandoml5，dividedinto4gr

2、oups：controlgroup(C)?andimmediatelyafterexhaustiveexercisegroup(Exhaustiive，EO)，24hgroup(E24)，48hgroup(E48)．Theone-timeexhaustiveexercisegroupperformedanexhaustivedownhillrunningtoexhaustionstate．Theexhaustiveexercisegroupandthecontrolgroupwereanesthetizedwith20％urethanebyanintr

3、aperitonealinjectionatdifferenttimepointsafterthelastexhaustiveexerciseandinaquietstate．5mlbloodssamplewastakenfromtheabdominalaortatomakeserum，andtheserumwasusedtomeasurethehepatocytegrowthfactorcontents．Asideoftherectusfemorismusclesamplewasfixedwith2．5％glutaraldehydeforproduc

4、ingultrathinsectionsusedtoobservetheultrastructuralchangesbytransmissionelectronmicroscope。TheothersidewasreservedinliquidnitrogentomeasurethehepatocytegrovnhfactorcontentsAnother4adultratswasdividedinto4groups：controlgroup，Immediatelyaftercontusiongroup(Contusion．DO)。24hgroup(D

5、24)，48hgroup(D48)．Atdifferenttimepointsaftercontusion，asideoftherectusfemorismusclesampleswerefixedwith2．5％glutaraldehydeforproducingultrathinsectionsusedtoobservetheultrastructuralchangesbytransmissionelectronmicroscope．Theothersideofthevastuslateralismuscleforcultureofsatellit

6、ecells．Anotherneonatalrat‘SvastuslateralisalsousedforcultureofmusclesatellitecellsResults：1)Afterofle—timeexhaustiveexercisejexhaustiveexercisegroup‘s(E0，E24，E48)skeletalmuscleultrastructuralstructurewassiginificiantlychanged：@Themyofibrilbecamethinnerandmoreincompact．HbandandIb

7、andtookill—defined．MlinesandZlinesblurred．Zlineswerefloweddistortedanddisappeared；@Musclegapwidenedwithaninterstitialedema；@Endoplasmicreticulumslightlydeformed．oreventwisted；@Somematrixbecomeshallow，andmitochondrialswereobviouslydeformed，distortedandevenswollen．Withtimeextendin

8、g，thedamageoftheexhaustiveexercisegroup’Smuscle