tgev双抗体夹心elisa方法建立与胶体金免疫层析试纸条的制备

《tgev双抗体夹心elisa方法建立与胶体金免疫层析试纸条的制备》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、东北农业大学农学硕士学位论文3．1ResultofPurificationofAscites⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．203．2ResultofPreparationandPurificationofPolyclonalAntibodies⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯203．2．1ResultofExpressionandpurificationofTGEVNProtein⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．203．2．2ResultofExpressionandpurificationofTGEVNProtein⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．223

2、．3ResultsofpreliminaryapplicationoflysatonthedoubleantibodysandwichELISA⋯．．．．．．．．．．223．4ResultofdevelopmentandoptimizeofadoubleantibodysandwichELISA⋯⋯⋯⋯⋯⋯⋯⋯一233．4．1Determinationoftheoptimumreactionconditions⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．233．4．2DeterminationoftbestandardELISAiudgment⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯233．5

3、ResultsofthepreliminaryapplicationoftbedoubleantibodysandwichELISA⋯⋯⋯⋯⋯⋯．．243．5．1Resultsofspecificityex脚mems⋯⋯⋯⋯．⋯⋯⋯⋯⋯．．243．5．2Resultsofsemitmtyexpefj曲嘲}I

4、s⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．．243．5．2Resultsofl’epea蜥陆Iyexpefi帕删b⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯一．：z53．5．3ResultsofcomparationoftheDoubleantibodysandwichELI

5、SAandRT-PCR⋯⋯⋯⋯⋯253．6QualityCertificationofAntibodyLabelbyColloidGold⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯一263．6．1QualityCertificationofColloidalGoldParticle⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．263．6．2QualityCertificationofAntibodyLabeledbyColloidGold⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯273．7DeterminationofPreparationofAntaib‘xtyMarkedbyColloidGold⋯⋯⋯⋯⋯⋯

6、⋯⋯⋯⋯⋯⋯⋯273．7．1DeterminationoftheAddingAmoumofSodiumCitrate．．3．7．2DeterminationoftbeOptm曲pH⋯⋯⋯⋯．3．8SelectofOvtimalReactionCmalitionsofKit．．．．⋯⋯⋯⋯⋯⋯⋯⋯．．27⋯⋯⋯⋯⋯⋯⋯⋯一⋯⋯⋯．．．283．8．1Sckx皤i蛐ofNCM⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．．273．8．2SelectionofGCM⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯一273．8．3SelectionofSample

7、padandabsorbentpad⋯⋯．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯283．8．4SelectionofBlockingagentandclosingtime⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．293．8．5SelectionofGoldstandardantibodydilutionfactor⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．303．8．6ResultofSpecificity⋯⋯⋯⋯⋯⋯⋯．⋯．⋯⋯⋯⋯⋯⋯．⋯．．⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．303．8．7ResultofStability⋯⋯⋯⋯⋯．303．8．8ResultofRepetit

8、iveness⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．3()3．8．9ResuRofclinicalsampletest⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯3(I’3．9Resultsofthe1019iIiIlIii洳of谪-alproteinlysateandapplication011theteststrip⋯⋯⋯⋯⋯．．343．9．1R