百合杂交育种与农杆菌介导的遗传转化初步分析

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1、百合杂交育种及农杆菌介导的遗传转化初步研究AbstraCtLily，akindofPerennialbulbPlantwithlargeandeolorfulflowers，hasbeenoneofthemostmPortantfresheutflowers.Reeentyears，PrimarystudyforvarietyimProvement15revolvingthebreedingobjectives，suehasornamentaleharaeter，fastness，qualityandreProduetivecapaeity.ThisstUdy15akindofPrelimin

2、aryexPlorationforvarietyimProvementoflilybyapProaehingoferossbreedingandgenetietransformation.1.TheimPactfactorsineludingsueroseeoneenirationandstoragetimeforinvitrogerminationweretested.Theresultsrevealedthat100g/LsueroseliquidmediumsuPPlemeniedwith2mg/Lborieaeidand10mg/LCaC12wassuitableforPollen.e

3、ulture.PollensfromL.long沪orumand‘Gelria，belongingtoLongiflorumhybrids，‘MareoPolo’，‘Bemini，，‘AcaPuleo，and‘Novatore，belongingtoOrientalhybrids，‘BrightYellow，，‘YellowGiant，andL.davidiivar.unicolorbelongingtoAsiatiehybrids，weretestedwithhighPollenviabilityandeouldbetheapProPriateehoiceforPaternalParents

4、.‘Ieeberg，fromAsiatie场bridsand‘Tiber，fromOrienialhybridsweretestedwithlowPollenviabilitybutsuitableformatemalParents.TheviabilityofthePollenfromL.davidiivar.unieotorandL.fong必rumeouldlastfor20duPondryingPreservationat4℃.2.ThedeveloPmentofPollenonstigmasuxfaceindifferenthybrideombinationswasobservedb

5、yfluoreseeneemieroseoPy.ThePollengerminatesandgrowsinstigmainshybrideombinations，ineludingL.long沪orumx‘Biaritz，，‘SimPlon，x‘Ieeberg，，‘Biaritz，XL.davidiivar.unicolor，‘BrightYellow，X‘Gelria，，‘Gelria，XL.davl’diivar.unicolor，whiehshowedeomPatiblenessbetweentheParents.3.Theilnnlatureembryosof38hybrideombi

6、nationswereeultUredinvitro.ThetoPgerminationratewasfoundinBSmediumsuPPlementedwithg%sucroseWhenovulesfrominbreedingofthewildlilyinGuizhouwereeultUred.ItwasalsofoundthatBSmediasuPPlementedWith6%org%sueroseweresutableforthedeveloPmentofovuleininitialstageofovaryslieeeulture.TheovarywasPiekeduPforsliee

7、eultUreandovuleeultUreafter25dofthestubstylePollination.Onehybridof‘SimPlon，X‘Siberia，wasfoundintheBSmediumsuPPlemeniedwithg%suerose，andthehybridwasidentifiedbasedonSequence一relatedamplifiedpolymorphi