塞来昔布联合x线对人胆管癌细胞株qbc939凋亡的影响

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1、青岛大学硕士学位论文塞来昔布联合X线对人胆管癌细胞株QBC939凋亡的影响姓名：王阳申请学位级别：硕士专业：肿瘤学指导教师：沈方臻20120602中文摘要塞来昔布联合X线对人胆管癌细胞株QBC939凋亡的影响目的研究塞来昔布联合X线对人胆管癌细胞株QBC939凋亡的影响并对其机制进行初步探讨。方法CCK一8法检测不同浓度的塞来昔布及不同剂量的X线对QBC939细胞株的增值抑制率，确定塞来昔布组的IC50及X线组的ICso。再将QBC939细胞株分为5组：对照组、单纯X线组(R)、单纯塞来昔布组(C)、塞来昔布先于X线组(C+R)，X线先于塞来昔布组(R-屺)。用流式细胞仪检测各组的凋亡率

2、，westernblotting检测凋亡相关基因survivin蛋白的表达。结果联合使用塞来昔布和放疗组的凋亡率有明显的增加，从8．268％，11．233％到15．733％，22．133％伊

3、heapoptosiseffectofcelecoxibincombinationwithx—rayonhumancholangiocarcinomacelllineQBC939Abstractobjective111ispresentstudymainlyaimedtoinvestigatetheproliferationandapotosiseffectsofcelecoxibincombinationwitIlX-rayonhumancholangiocarcinomacelllineQBC939andthepossiblemechanisminvolved．MethodsFirs

4、t，CellCountingKit-8(CCK-8)assaytoinvestigatetheinhibitiveratesafterthetreatmentofcelecoxibandx—ray．WedeterminedtheIC50byCCK-8assay，whichWasadoptedinthefollowingexperiments．Thenthecellsweredividedintofivegroups：controlgroup，x-raygroup，celecoxibgroup，x-rayafterthecelecoxibgroupandX-raybeforecelecox

5、ibgroup．Finally，apoptosisofeachgroupweredeterminedbyflowcytometry，theproteinlevelsofapoptotic-associatedgenesurvivinWasdeterminedbywestemblotting．ResultsTheapoptosisratesafterthecelecoxibincombinationwithx—raywereenhancedapparentlyto22．133，15．733％from8．267，11．233％(P

6、ingshowedthatsurvivinproteinexpressionWasdown-regulatedobviouslybycelecoxibincombinationwithx-ray(P

7、elecoxibmayreducetheexpressionofsurvivinoracceleratethedegradationofsurvivinprotein．Postgraduatestudent：YangWang(Oncology)DirectedbyProf．Fang—ZhenShen[Keywords]Cholangiocarcinoma；eeleeoxibsx·raylsurvivin；apoptosis．引言胆管