内质网应激相关基因在自身免疫性疾病中的表达

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1、目录英文缩略词表............................................................................................1中文摘要....................................................................................................4英文摘要...............................................

2、.....................................................71前言......................................................................................................112实验材料...........................................................................................

3、...122.1动物...................................................................................................122.2菌株、细胞株及质粒........................................................................122.3主要试剂.....................................................

4、......................................132.4引物及siRNA...................................................................................142.5主要仪器与设备................................................................................153实验方法........................

5、......................................................................163.1主要试剂溶液的配方.......................................................................163.2临床样本收集及处理.......................................................................173.2.1健康人及SLE、RA

6、及AS病人外周血样收集..........................173.2.2外周血白细胞分离........................................................................173.3重组质粒的构建及质粒提取...........................................................183.3.1引物设计及合成...........................................

7、.................................183.3.2样本RNA的提取、定量.............................................................183.3.3RT反应cDNA的制备..................................................................193.3.4常规PCR以及产物回收...........................................

8、...................193.3.5凝胶DNA片段的回收.................................................................203.3.6DNA连接.......................................................................................2