银杏叶对脑缺血再灌注损伤的神经保护作用与机制

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1、山东大学硕士学位论文examinedbyinsituend-labelingoffragmentedDNAusingtheterminaldeoxynudeotidyltrausferasereaction(TUNEL)．IPPimageanalysissystemwasusedtOanalyzetheresults．Inaddition，weabservedthehistologicandpathologicchangesincodexandcorpusstriatumofbrainsectionbycommonmicroscopeandelectronicmicroscope．Resul

2、tsI．Neuronicapoptosis：Therearefewapoptosiscellsinshamoperationgroupsincodexandcorpusstriatum．Comparedwithshamgroup，apoptosiscellsincreasedsignificantly(P<0．01)inischemic—reperfusiongroupsat6-hourpoint，andthemostat2-daypoint．Thecellslocatedmoreincorpusstriatumthancortex(P<0．05)．Theapoptosiscellscoul

3、dbefoundatvascularendotheliumcellsofbraintissueandvenationclusterepitheliumcellsofside·ventricleexceptforneurons．Therearelotsofapoptosiscellsandapoptosisbodysbetweenfulldeathareasandnormalareasaroudofischemiazonesat4-daypointandtherewasnoTUNELstaininfulldeathareas．Therewasnosignificantlydifferenceb

4、etween2-dayand4-daygroupsafterreperfution(P>0．05)．EGbcouldn’tpreventtheapoptosisandthenumberofapoptosiscellsincreasedsignificantlythanshamgroup(P<0，01)．ButEGbinhibitedsignificantlythecellapoptosisafterbrainI／R(p<0．01)．2．ThepositiveespressionsofCD54andNOS：Immunohistochemistryresultsshowedthattheposi

5、tiveespressionsofCD54andNOSinmicrovascularendothelialcellswereobservedincortexandcorpusstriatumofallgroups．Butintheshamoperationgroupstherewereonlyafewones．Ascomparedwithshamgroup，theexpressiongradesofischemic·reperfusiongroupsandEgbgivengroupswerebothsignificantlyincreased(p<0．01respectively)，espe

6、ciallyat2-daypoint．AndthenumberofpositiveexpressioncellsinEgbgivengroupswaslowerthanthatofIIgroup(p<0．O1)3．ThepositiveespressionsofNRlmRNA：InshamoperationgroupstherearefewNRlmRNApositivecells．butinthelatter2groupsthenumberofpositine—‘．山东大学硕士学位论文cellsincreasedsignificantly．ThepositiveespressionofMCA

7、OgroupsandEgbgivengroupsbothdistributedmainlyinbraincortexandcorpusstriatum．ThenumericaldensityandtheopticaldensityofNRlmRNApositivecellsinMCAOgroupsdecreasedsignificantlycomparedwithEgbgivengroups(p<0．01)．