腭裂骨膜牵张成骨修复与机理的初步分析

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1、遵义医学院硕士毕业论文雳裂骨膜牵张成骨修复与机理的初步研究periostealdistractiondevicebelongtoexperimentalgroup，andtheotherswithoutimplantationbelongtocontrolgroup．Therest2dogsthathavenotbeenoperatedbelongtonormalcontrolgroupandwillbekilledonthe60thday．埘cottetracycline(50mg／kg)intramuscularlyintothedogsonthe14thdaya

2、ndthethirddayseparatelybeforetheyarekilled．Applythehardtissueslicingtechnologyinharvestingasmallboneof0．5cmxlcmrespectivelyfromthenewlyformedboneintheexperimentalgroup，andfromcontrolgroupandnormalcontrolgroup，andmakeahistologicalanalysisonthemandexaminethebonetissueinmetrologicaldynamic

3、parameter，SOastOelucidatetheregularityofnewboneformation．Results：(1)PT-PCRexperimentalresults：asmallamountofACTAandFSmRNAaredetectedonthe10thdayto60thdaybonetissuesamples，inwhichthelateralboneatthesectionhasnotbeendistracted，andthecomparisonamongthemareinnostatisticalsense(p>0．05)．ACTAa

4、ndFSmRNAbegintoexpressinthenewlyformedboneatthedistractedsidel0daysafterfixationofperiostealdistractiondevice．AndtheexpressionamountofACTAandFSmRNAincreasesgraduallyalongwiththebeginningofimplantingperiostealdistractiondevice．The’expressionamountsofACTAandFSmRNAatthe20th，30th，40m，and60t

5、hdayafterdistractionare1．78times，2．82times，4．27times，and3．04timesasmuchasthatofthe10thdayrespectively．ThedifferencebetweentheexpressionamountoftheACTAmRNAinthe10thdaysampleandthatinthe20thdaysamplehasnostatisticalsignificance(p>O．05)；Thedifferencesbetweenthelothdaysample，the30thdaysampl

6、e，the40山daysampleandthe60恤daysamplehavestatisticalsignificance(∥0．05)；thedifferencebetweenthe20tlldaysampleandthe30mdaysamplehasstatisticalsignificance(p>0．05)：thedifferencesbetweenthe20mdaysample，the40mdaysampleandthe60thdaysamplehavestatisticalsignificance(p<0．05)：thedifferencesbetwee

7、nthe30mdaysample，the40mdaysampleandthe60thdaysamplehavestatisticalsignificance(∥，0．05)：thedifferencebetweenthe40thdaysampleandthe60mdaysamplehavestatisticalsignificance(∥O．05)．ThedifferencebetweentheexpressionamountsofFSmRNAatthedistractedsideinthel0tlldaysampleandthatinthe20th