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1、中国修复重建外科杂志2008年2月第22卷第2期·145·组织工程骨膜异体体内成骨修复兔骨缺损的初步观察123311赵琳史志勇周晟贾有福刘佳王军胜【摘 要】目的探讨以兔BMSCs和猪SIS复合构建的组织工程骨膜，在异体兔体内成骨的可行性。方法取新西兰大白兔BMSCs与SIS复合构建组织工程骨膜。选2月龄新西兰大白兔12只，制备双侧桡骨1.5～2.0cm缺损模型。随机选一侧植入组织工程骨膜,作为实验组；另一侧仅植入单纯SIS，作为对照组。术后观察动物一般情况，4周后摄X线片观察，取骨缺损中段标本行HE及Masson染色观察。结果
2、两组动物术后饮食及日常活动基本正常；伤口无红肿、溢脓等；伤肢基本能负重行走。X线片观察：实验组骨缺损处有长条状新生骨形成，密度与正常骨相同，新生骨桥接骨缺损；对照组骨缺损无骨形成征象，骨缺损处密度与周围软组织影相近。组织学观察：实验组骨缺损处有新骨形成，骨组织中可见血管腔及不规则髓腔样结构；未见明显异物巨噬细胞及淋巴细胞浸润征象；对照组骨缺损处仅为胶原瘢痕组织，无骨组织形成。结论以SIS和BMSCs构建的组织工程骨膜在同种异体体内可以成骨，有修复骨缺损的可行性。【关键词】 组织工程骨膜 BMSCs SIS 骨缺损兔中图
3、分类号：R318.08 Q813 文献标志码：ATHEPRIMARYOBSERVATIONOFTISSUEENGINEEREDPERIOSTEUMOSTEOGENESISINVIVOINALLO-GENICRABBIT/ZHAOLin1,SHIZhiyong2,ZHouShen3,JIAYoufu3,LIUJia1,WANGJunsheng1.1DepartmentofOrthope-dics,the2ndhospitalofLanzhouuniversity,LanzhouGansu,730030,P.R.China;2Th
4、eExperimentalAnimalCenterofGeneralHospitalofLanzhouMilitaryRegion;3DepartmentofRadiology,ChinesetraditionalMedicinehospitalofGansuProvince.Cor-respondingauthor：ZHAOLin,E-mail：bonezl@sina.com【Abstract】Objective Toinvestigatetheinvivoosteogenicfeasibilityoftissueengin
5、eeredperiosteumconstructedbyporcineSISandBMSCsinallogenicNewZealandrabbit. MethodsThetissueengineeredperiosteumconstructedbySISscaffoldandBMSCswaspreparedinvitro.Twelve2-month-oldNewZealandrabbitswereusedintheexperiments.The1.5-2.0cmcriticalbonedefectsweremadeintheb
6、othsidesofradiusoftheanimals.Thetissueengineeredperiosteumwasgraftedintoonesidedefectrandomly,whiletheothersidedefectwasonlygraftedSIS.Fourweeksafteroperation,theforearmsofallanimalswerecheckedbyX-ray.Then,animalsweresacrificedtoharvestthespecimenwhichweretreatedpro
7、mptlyforHEandMassonstaining.TheX-rayfilmandthemorphologicaltissuestainingoutcomewereevaluatedqualitatively. Results Afteroperation,allanimalshadanormalbehavioranddiet;theincisionhealednormally;theforearmcouldmovenormallyforbearingweight.Thetissueengineeredperiosteum
8、constructedbyallogenicBMSCsandheterogeneicSISscaffoldcouldformnewbonetissue,andbridgedthebonedefectwhichcouldbeconfirmedeitherinX-rayfilmo
