缺血再灌注大鼠脑微血管损伤机制分析

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1、缺血再灌注大鼠脑微血管损伤机制研究microvascularimpairmentinischemicstroke．PartITherelationshipbetweenRho—kinaseandmicrovascularimpairmentobjective：ToobservetherelationshipbetweenRho-kinaseandmicrovascularimpairmentbymiddlecerebralarteryocclusion(MCAO)model．Methods：AllmaleSDratsboughtfromthelaboratoryan

2、imalscentreinouruniversitywererandomlydividedintofollowingfivegroups：shamgroup，MCAO6hours，24hours，48hoursand72hoursgroup．TherearefifteenSDratsineverygroups．Therightmiddlecerebralarteryischemia--repefusionmodelsdescribedinitiallybyZea·-Longawereestablished．After90minutesoftemporaryMCAO，

3、Theratswererespectivelysubjectedreperfusionof6h，24h，48hand72h．InthispartofexpefienttheexpressionlevelsofRho—kinasemRNAweredetectedbyRT—PCR．AndwekilledtheratsanddrawtheischemicpenumbrabraintissueofinfarctsidetoobservethechangeinpermeabilityofBBBbydetectingthecontentofEvan’Sblue(EB)，tome

4、asuretheexpressionlevelsoflamininbyimmumofluores-cencemethodandtoobservetherelationshipbetweenthem．Results：Thereareobviousstatisticsdifference(P<0．01)betweentheischemia-repefusion6hours、24hours、48hours、72hoursgroupsandshamgroupintheneurologicalfunctionaldecect；AmongthefourMCAOgroupsthe

5、neurologicalfunctionaldececthavenostatisticsdifference(P>0．05)．TheexpressionlevelsofRho—kinasemRNA、thecontentofEBandtheexpressionleveloflamininhaveobviousstatisticsdifferencebetweenthefivegroups(P<0．05)；Thereareobviousstatisticsdifference(P

6、ercerebralischemia-repefusion，theexpressionlevelsofRho-kinasemRNAandthecontentofEBbegintoincreaseatthetimeof6handwillgetitspeakatthetimeof48handdecreasegradually48hlater．Theexpressionleveloflamininbegintodecreaseatthetimeof6handwillgetitsminimumatthetimeof48handincreasegradually48hlate

7、r．TheexpressionlevelsofRho—kinasemRNApresentspositivecorrelationwiththecontentofEB，r=0．925，P=O．025<0．05；TheexpressionlevelsofRho—kinasemRNApresentsnegativecorrelationwiththeexpressionleveloflaminin，r=-0．955，P=O．011d0．05；ThecontentofEBpresentsnegativecorrelationwiththeexpressionlevelo