骨髓冲洗去除对骨形成影响的实验分析

《骨髓冲洗去除对骨形成影响的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、EXPERIMENTALSTUDYOFTHEEFFECTONBoNEFORMATIoNAFTERⅣL▲RROWABLATIONABSTRACTObjective：TheboneformationiSacomplicatedprocesswithmanykindsofcellsandmutiplefactorparticipating．Theosteoblastsrecruitandduplicate，andgetdeeplydifferentiatedwhichrusultsinthenewboneformationthroughtheaccum

2、ulationandmineralizationoftheextracellularmatrix．111isprocessisinfluencedbymanyfactors．InChina，thepopulationaginghasbeenbecomeasocialploblem．Foroldmen，theosteoporosisinducingfractureswouldbeincreased．Howtopreventandcureosteoporosisandpromoteboneformationisaploblemtobesolvedwh

3、ichpossessesbigclinicalsignificance．Itisknownthatbonemarrowplaysanimportantroleintheprocessofboneformation．It’Shelpfulinevaluatinginvivoboneformationandresorptioninarelativelyshorttimespan．Somereseachershavereportedtheoaeogenesisisenhancedfollowingmarrowablation．Accordingtoth

4、istheory，wedesignedthisexperimenttoobserveandapproachthebonegenerationchangingpreliminarilyaftermarrowablationthroughobservingofX—raymean伊ayvaluesandbiomechanicsparameters．Theobjectiveofourstudyistoforseetheoperationmethodforcuringandpreventingosteoporosis．五英文摘要Methods：Weempo

5、lyedthirty-sixfamaleSpragueDawleyrats，eachweighingapproximately3009．Theyweredividedintotwogroupsofeighteeneachinrandom．Thetwogroupsarenormalsalineirigatinggroup(NSgroup)andVitD3irigatinggroup(VDgroup)．Animalswereanesthetizedwithketamine(100mg／kg)byintraperitonealinjection．A1．

6、0cmincisionwasmadeoverthekneejointandtheintracondylarnotchwasexposedundersterileoperation．TheintracondylarnotchwasopenedbydrillingfollowingthemarrowcavityinuseofI．OmmdiameterKirschnerwire．TheleRlowerextremityofNSgroupanimalsisexperimentalgroupside(EGside)andsubjectedtobonemar

7、rowablationbynormalsalineflushing．Theoppositesideisshamexperimentalgroupside(shamside)illwhichanaccessholeWasdrilledinthefemoralintracondylarcontexwithoutremovalofthebonemarrow．Thentheincisionwasclosedinfull-thickness．TheleftlowerextremityofVDgroupanimalsisexperimentalgroupsi

8、de(EGside)，inwhichthemarrowcavityisflushedbyVitD3．Themarrowablationo