大鼠肺纤维化模型的微型ct分析

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1、■●andscannedwithmicro．CTforradiologicstudies．Managementofspecimensandscanprotocolwerereferredtothefirstpart．TherightlungswereexaminedhistologicallywithHEstain，massontrichromestainandimmunohistochemicalstain．Alveolitisandpulmonaryfibrosiswasquantified．Observa

2、tionsofthoracicimagingincludedground—glassattenuation，consolidation，linearopacities，nodules，tractionbronchiectasis，subpleuralinterstitialthickening，bullas，pneumothoraxandhoneycomblung．Imagingfindingsinvivoandaftersacrificeatmicro．CTwerecompared．Pulmonarychan

3、gesoffoursub-groupsaftersacrificewereevaluatedandcomparedwithcontrolgroup．Imagesofspecimenswereinvestigatedandcomparedwithimaginginvivoandaftersacrifice．AppearanceofradiologicsignsindifferentstagesofPFWasalsocompared．Results1．Appearanceofconsolidation，bullas

4、，pneumothoraxandhoneycomblungofima西nginvivoandaftersacrificewasclosetoeachother．Appearanceofground—glassattenuation，nodules，subpleuralinterstitialthickeningandtractionbronchiectasisofimaginginvivowasunderitofimagingaftersacrifice，butthedifferencehadnostatist

5、icalsignificance．ImagingaftersacrificeWasgoodatdisplayinglinearopacities(P

6、ofappearanceofground—glassattenuationandconsolidationhadnostatisticalsignificanceinsub—groups．Nodulesandsubpleuralinterstitialthickeningdidnotappearin3dgroup．Honeycomblungandtractionbronchiectasisappearedin28dgroup．3．Specimenimagingwasclearerthanimaginginviv

7、oandaftersacrifice．4．HEstainandmassontrichromestainsuggestedthatitWasmoderateorseverealveolitismainlyinexperimentalgroupof3dand7danditWasmoderateorseverepulmonaryfibrosismainlyin14dand28d．5．Immunohistochemicalstainshowedthatpositivecellsinlungsofexperimental

8、groupwereincreasedandintensityofstainingwasboosted，particularlyin7dgroup．Positivecellsin28dgroupwerelessthanthosein7dgroup，butwerestillsignificantlyhigherthanthoseincontrolgroup．6．Linearopacitie