大鼠ami后急性高血糖对心肌细胞线粒体膜电位的影响

《大鼠ami后急性高血糖对心肌细胞线粒体膜电位的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、徐州医学院硕士学位论文EffectofAcutehyperglycaemiaonMitochondriaMembrancePotentialafteracutemyocardialinfarctioninratsAbstractObjectiveHyperglycaemiaisverycommoninthepatientswitllacutemyocardialinfarction(AMI)inclinic．ManyclinicalobservationshavedemonstratedmathyperglycaemiaCanobvious

2、lyenlargemyocardialinfarctionsize，increasethemortalityandaffectetheprognosisofthepatientswithAMI．ApoptosisWasbelievedtobeanimportantwaythatcardiacdiedafterAMI，anditmarkedlyaffectedmyocardialinfarctionsize，theventricularfunctionandtheprognosisofthepatientswithAMI．Thepresent

3、studyaimedtoobservetheeffectofacutehyperglycaemiaonapoptosisanddiscusseditspossiblemechanismbyreproducingtheanimalmodelofAMlwithhyperglycaemia．MethodsThemodelofAMlwasreproducedbyligatingtheleftanteriordescendingcoronaryartery．Then，theforty-sevenmaleSDratswererandomlydivide

4、dinto4groups：controlgroup：theratsweretreatedbyintravenousinfusionofphysiologicalsalineafterAMI；HGlgroup：theratsreceivedintravenousinfusionof25％glucosesolutionafterAMI；HG2group：theratsweretreatedbyintravenousinfusionof50％glucosesolutionafterAMI；Insulingroup：theratsreceivedi

5、ntravenousinfusionof50％glucosesolutionafterAMIand5unitregularInsulinWassubcutaneoulyinjectedatonce．AnothertenmaleSDratsweresupposedasshamgroup：theoperationissimilarexceptforligatingtheleftanteriordescendingcoronaryarteryandthephysiologicalsalinewasintravenouslyinfusedafter

6、theoperation．Thefluidwasinfusedbyapumpatthespeedof4ml／kg’h，continuouslyfor30minineverygroup．BloodglucoselevelsWL。l-emonitoredthroughouttheexperiment．Attheendoftheexperiment，themyocardialsampleswastakentomeasuretheindicators．Thehistopathologicalchangesofhearttissueswasobese

7、rvedwithHEstainingbylight·-microscopy；theexpressionofcaspase·9wasmeasuredbyimmunohistochemistry；theapoptoticindex(AI)andMitochondriaMembrancePotentialwasdeterminedbycorrespondingkitseparately．TheexpressionofCyt-Cinmitochondriaandendochylemaweredeterminedbywestemblotting．5◆

8、僖●，一徐州医学院硕士学位论文Results1．ThemodelofAMlwithhyperglycaemiainratswassuccessfullybuiltedbyliga