不同慢性疾病中的心磷脂代谢变化与意义

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1、（3）荧光定量PCR法检测所收集的右心耳心肌组织中ALCAT1mRNA的表达。结果：（1）B组（T2DM并风湿性心脏病组）与A组（风湿性心脏病组）相比较，B组ALCAT1mRNA表达量大于A组，存在着显著差异（A组0.029＋0.005；B组0.039＋0.004；P＜0.05）。（2）D组（T2DM并冠心病组）与C组（冠心病组）相比较，D组ALCAT1mRNA表达量大于C组，存在着显著差异（C组0.036＋0.005；D组0.043＋0.004；P＜0.05）。（3）C组（冠心病组）与A组（风湿性心脏病组）相比较，C组ALCAT1mRNA表达量大于A组，存在着显著差异（A组0.029＋0.0

2、05；C组0.036＋0.005；P＜0.05）。结论：（1）2型糖尿病、冠心病存在着心磷脂代谢障碍。（2）2型糖尿病与冠心病的叠加，心磷脂代谢障碍愈严重，其分子机制是心肌中ALCAT1mRNA的高表达。关键词：糖尿病；心磷脂酰基转移酶1；心磷脂病态重构IIABSTRACTBackgroundDiabetesmellitusisakindofmetabolicdisorderwhichischaracterizedbychronichyperglycemiaduetoavarietyofpathogenesis.Butthecardiovasculardiseasecausedbytype2d

3、iabetesmellitus,especiallycoronaryheartdiseaseisthemaincomplicationsandcausesofdeath.Diabetesmellitus,obesityandcoronaryheartdiseasehaveacommonpathologicalfeature,forexempleoxidativestressandmitochondrialdegeneration,etc.Cardiolipinmetabolismdisorders,docosahexaenoicacidincardiolipinenrichedinmitoch

4、ondrialedtomitochondrialdysfunction,whichhaveattractedtheattentionofdomesticandforeignexpertsandscholarsonmitochondrialdegeneration,cardiolipinmetabolismandtheenzymeofregulatingcardiolipinremodeling.Inrecentyears,Acyl-CoA:lysocardiolipinacyltransferase-1ofregulatingpathologicalremodelingofcardiolipi

5、nhasbecomethenewentrypointtorevealthepathogenesisofdiabetesmellituscausingcoronaryheartdiseaseandcardiomyopathy.Nearlyallthecardiolipinarelocatedintheinnermitochondrialmembrane.Themitochondrialisextremelyrichinthemyocardialcells,Cardiolipinremodelingisalsobelievedtoplayanimportantroleinrepairingdama

6、geofthefunctionofmyocardialcellsfromoxidativestress.Studiessuggestedthatpathologicalcardiolipinremodelinghashappenedtopatientswithdiabetesmellitusandobesity.Inaddition,aberrantCLspecieswerecommonlyfoundinthemyocardiumofpatientswithcoronaryheartdisease,ALCAT1isakeyenzymethatregulatespathologicalremod

7、elingofcardiolipinandleadstoaberrantCL.ALCAT1ismainlylocatedatthemitochondria-associatedERmembrane,MAMisamembranebridgebetweentheERandmitochondria,whereALCAT1expressionlevelisthehighestinheartandliver