穴位埋线对卒中后大鼠痉挛的抑制作用与乙酰胆碱酯酶的影响

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1、穴位埋线对卒中后大鼠痉挛的抑制作用及乙酰胆碱酯酶的影响经两独立样本t检验统计分析，P=0.035，P<0.05，两组之间差异有统计学意义；5．脑干中乙酰胆碱酯酶活性的测定：治疗组和模型组数据采用秩和检验，P=0.001，P<0.05，两组之间差异有统计学意义。结论1．线栓质量不合格，干预手段操作不当，可能是引起大鼠死亡和模型不成功的主要原因；2．穴位埋线治疗能够改善缺血性脑卒中后痉挛大鼠肢体肌张力；3．穴位埋线能够提高缺血性脑卒中后肢体痉挛大鼠的脑干中乙酰胆碱酯酶活性，降解乙酰胆碱，使肌肉兴奋性降低，肢体痉挛逐步缓解。关键词穴位埋线；脑卒中；肌痉挛；乙酰胆碱酯酶-2-ABST

2、RACTEffectofInhibitionandtheAChEActivityofCatgutImplantationatAcupointonRatsofSpasmafterStrokeAbstractObjectiveCerebralarterialthrombosismodelratswithspasmastheresearchobject,toobservetheeffectofcatgutimplantationatacupointfortheactivityofAchEwhichchangesinthebrainstem.Andtoprobetheactionme

3、chanismofcatgutimplantationatacupointtreatment,CapsuleforInhibitingspasticity,ontheanimalmodelsofratswithcerebralarterialthrombosis,andtoprovidethefoundationtofortherapyofspasticityafterstrokeonclinic.MethodsSprague-Dawleymalerats(n=60)wererandomlydividedinto3groupsmakinguseofSAS9.2statisti

4、calanalysissoftware:thetreatmentgroup(25cases),themodelgroup(25cases),andtheshamgroup(10cases).Themodelratswiththemiddlecerebralarteryocclusion(MCAO)werepreparedaccordingtothemethoddescribedbyZeaLongamethod.Cerebralarterialthrombosismodelratswithspasmweretheresearchobject.Zealonganeuralfunc

5、tionevaluationandModifiedAshworthScalewereundertakentoevaluatetheresultofthemodel.TheinclusionconsistedofratsmodelofMCAO,theexclusionconsistedoffailmodelratsandthedeathintheprocessofmakingmodelandintervention.Theratsastheresearchobjectwereselectedatthelast,thetreatmentgroup(8cases),themodel

6、group(11cases),andtheshamgroup(10cases).Thetreatmentgroup:Catgutimplantationatacupointtreatmentwasadded5dayslaterafterthemodel,keepingon7daysatDazhui，Jizhong,Guanyuan,Zhongwan,Houhui.Then,2%pentobarbitalsaltwasintraperitoneallyadministered(40mg/kg).Isolatedtissueperfusionsystemwasusedtomeas

7、urethechangeofmuscletension,andImmunohistochemicalmethodwasusedtoobservetheactivityofAchEinthebrainstem,toevaluatethecurativeeffectofcatgutimplantationatacupointreleasingthespasmratsafterstroke.Themodelgroupwasnotreceivedanytreatment.Theshamgroupjustonly