丹参酮ⅱa对大鼠脑缺血再灌注后细胞凋亡、drp-1及caspase3表达的影响

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1、目录中英文缩略表..................................................................................3摘要................................................................................................4Abstract..........................................................................................61

2、前言............................................................................................92实验材料与方法..........................................................................92.1实验材料.............................................................................92.1.1实验仪器..................

3、..............................................102.1.2主要试剂................................................................112.2实验方法.......................................................................122.2.1大鼠脑缺血再灌注模型的制备..................................122.2.2丹参酮IIA的溶解...................

4、..................................152.2.3动物分组...................................................................152.2.4动物给药方式和药物剂量.........................................152.2.5大脑中动脉脑血流的检测..........................................152.2.6TTC法测定脑梗死体积.........................................

5、....162.2.7TUNEL检测神经细胞凋亡........................................162.2.8免疫组化检测Drp-1及Caspase3的阳性表达........162.2.9Westernblot检测Drp-1及Caspase3的含量表达.172.3统计分析.......................................................................183实验结果...........................................................

6、........................184讨论..........................................................................................245结论..........................................................................................26参考文献......................................................................

7、.................27附录1...........................................................................................291致谢..............................................................................................30综述............................................