白藜芦醇对ka诱导的癫痫模型大鼠抗癫痫作用及机制的初步分析

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1、安徽医科大学硕士学位论文表现一定的抑制效应。2．低剂量Res对KA诱导癫痫模型大鼠海马CAl和CA3a区锥体细胞死亡(neuronalcelldeath)具有显著的保护作用，同时对MFS的抑制比VPA更为显著。3．低剂量的Res对KA诱导的颞叶癫痫(TLE)模型大鼠的癫痫发生和发展具有较好的治疗效果。关键词白藜芦酵癫痫kainiteacid丙戊酸钠4安徽医科大学硕士学位论文ABSTRACT—o‘’』●●■⋯’‘’‘，grimal了studyOnantl—eDIleDSVeriectsandmichanism0t

2、ResverotralinKainicacid-inducedepilepsyratObjectiveTheratswereinducedtemporallobeepilepsy(TLE)byKainateacid(KA)injectedintothecentersiteofCA3regionoffighthippocampuswiⅡlthestereotaxictechnology．Thefollowingchronicpharmacologicalexperimentswerecarriedonthismo

3、del．Threelevelsexperiments(behaviormonitoring，intmcalvariumelectroencepholographyrecordingandhistologicalanalyses)wereusedtoevaluatetheefficiencyandanalysethemechanismofresveratrol(Res)inanti-epilepsyfield．Methods1．KA·inducedTLE．AdultmaleWistarrats(220—260ga

4、ndcleanstage)wereusedintheexperiments．Underchloralhydrate(350mg／kg,i．p．)anesthesia,ratswereplacedOilthestereotaxicapparatusand2．5p．1KA(0．04rwgpl)wasslowlyinjected(about10min)toCA3regionofrighthippocampus(4．0mmposteriortobregma，4．4mmlateraltothemidline，3．8nlm

5、belowdura)．TheneedleWasleftfor3minandtherats’scalpsweresutured．ThebehavioralprogressionofKA-inducedseizureswasscoredaccordingtoRacine’Sstandardclassification．Thoseratsthatcouldreachedatleasttheclass4or5seizureswereusedinthisstudy．Thesamevolumesofnormalsaline

6、tothesanlesitewereinjectedinthecontrolgroup．2．Grouping．Alltheratsintheexperimentsweredividedrandomlyintothefollowingfivegroups：NormalSalinefNs)group，KAandVehicleSolution(VS)group，KAandSodiumValproate(VPA，300mg／kg／d)group，KAandlowdosageofRes(LRes，15mg／kg／d)gr

7、oup，andKAandhigIldosageofRas(I-IRes，30mg／kg／d)group．Thedrugswerefilledintostomachonceadayafterthefirstonsetofseizure，and安徽医科大学硕士学位论文continuancetendays．3．Behaviormonitoring．TwoweeksafterKAinjection，thespontaneousseizuresnumberofratsWaSrecordedin2monthes(8h／d，

8、5d／week，40h／week)byvideoCamera．4．Intracalvariumelectroencepholographyrecording(IEEG)．Afterthebehaviormonitoring，underchloralhydrate(350mg&g，i．p．)anesthesia，twoelectrodesofstainlesssteelforrecord