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1、中国组织工程研究与临床康复第14卷第1期2010–01–01出版JournalofClinicalRehabilitativeTissueEngineeringResearchJanuary1,2010Vol.14,No.1离心力对兔骨髓基质细胞成骨分化的影响*徐南伟，张煜，周栋，孙荣彬EffectofcentrifugalforceonosteoblasticdifferentiationofbonemarrowstromacellsinvitroXuNan-wei,ZhangYu,ZhouDong,SunRong-b
2、inAbstractDepartmentofBACKGROUND:Centrifugalforceisacontributingfactorinducingosteoblasticdifferentiationfrombonemarrowstromacells.Orthopedics,OBJECTIVE:ToexplorewhethercentrifugalforcepromoteosteoblasticdifferentiationfromrabbitmarrowstromacellseededonChangzhouS
3、econdpolylactic-co-glycolicacid(PLGA)scaffolds.People’sHospitalofMETHODS:Rabbitbonemarrowstromacellswereisolatedandculturedbywholebonemarrowmethod,purifiedbyattachmentNanjingMedical9University,method,anddigestedbytrypsin-EDTAat80%confluency.Thecellconcentrationwa
4、sadjustedto1×10/L.PLGAwascutintoChangzhoupieces,5mm×5mm,soakedinserum-conditionedculturemediumfor24hours.Thethirdpassageofbonestromacellsuspension213003,Jiangsuatadensityof300µLwasrespectivelyseededintothePLGAmaterial.Thescaffold/cellcompoundwasplacedincentrifuge
5、tube,Province,Chinawithcellattheupperlayerandculturedinosteoblasticinducedmediumcontainingantiscorbicacid,β-sodiumglycerophosphate,XuNan-wei,Chiefanddexamethasonerespectivelyundercentrifugalforceevery12hours(1000r/minfor30minutes,relativecentrifugalforce132g)phys
6、ician,Associateandstaticcondition.Alkalinephosphataseactivity,osteocalcincontentandcalciumcontentaswellasobservationbylightprofessor,microscopywereusedtoevaluateosteoblasticdifferentiation.DepartmentofRESULTSANDCONCLUSION:After16daysofinvitroculture,thescaffoldso
7、fcentrifugalforcegroupwerecoatedbyOrthopedics,ChangzhouSecondmultiplayercellsandmineralizedmatrix,butonlyathinlayerofcellswereobservedonthescaffoldofcontrolgroup.ThePeople’sHospitalofcentrifugalforcesystemresultedinasignificantdecreaseinalkalinephosphataseactivit
8、yatday2(P<0.05)butsignificantNanjingMedicalincreaseatday4comparedwiththestaticculturecondition(P<0.05).Duringthewholeculturetime,osteocalcinsecretionUniversity
